免疫胶乳凝集试验测定尿微量白蛋白

Immuno - latex agglutination test for microalbumin in urine

目的 通过免疫胶乳凝集试验定性或半定量测定尿微量白蛋白。方法以人白蛋白致敏浚化聚苯乙烯胶乳，建立了人白蛋白免疫胶乳凝集抑制试验，结果通过选择适当的抗人白蛋白抗血清稀释度，将本试验的阳性阈值调节至60mg/L水平。用本法测定了94例临床尿液标本。并与全自动蛋白质分析仪上散射速率比浊法的结果进行了比较，两者的符合率达到93。6％。结论本法快速、简便、价格低廉，适合于临床上对尿微量白蛋白的常规化验和体检时的大规模筛选。

Objective To determine microalbumin in urine qualitatively or semiquantita-tively with inimuno - latex agglutination test. Methods Using human albumin to sensitize carboxy-lated polystyrene latex, an human albumin immuno - latex agglubination inhibition test was established . Results By choosing suitable liter of anti - human albumin antiserum, the positive threshold value of this test was adjusted to 60mg/L. 94 clinical urine specimens were determined by this method, and their results were compared with those of rate nephelometry on automated protein analyzer. The correlation of these two methods reached 93.6% .Conclusions Being rapid,simple and cheap, this method is fit to be used in routine assay of urine microalbumin and in large scale screen during physical examination.