随机扩增DNA多态性技术在肠球菌流行病学分型中的应用

The application of RAPD - PCR in epidemiological classification of enterococci

目的 探讨运用随机扩增DNA多态性技术对肠球菌流行病学分型的方法。方法 采用随机扩增DNA多态性技术对分离的肠球菌进行基因水平的同源性分析。结果 显示明显可见10～20条数的DNA指纹图谱,经RAPD、PHYLIP和Treeview分析软件分析,分为3个聚类群。结论 随机扩增DNA多态性技术对肠球菌感染流行监测是一个有效的方法。

Objective To approach into the method of random - amplified polymorphic DNA ( RAPD) - PCR in epidemidogical classification of enterococci. Method RAPD - PCR was applied to analyse the gene - level homogeneity of isolated enterococci. Result Ten to twenty bands DNA fingerprint maps were illustrated and divided into 3 clusters by RAPD,PHYLIP and Treeview analysis software. Conclusion RAPD - PCR technique is an efficient method in epidemiological investigation of enterococcal infection.