摘要
研究适当应激对衰老小鼠海马线粒体膜流动性的影响。Ｄ－半乳糖诱导的衰老小鼠同时进行游泳应激训练（１８℃，１５ｍｉｎ），连续６周后，以ＤＰＨ为荧光探针，检测小鼠海马线粒体膜粘滞系数，同时测定线粒体丙二醛含量的变化。衰老组小鼠海马线粒体膜流动性降低，ＭＤＡ生成增加，而１８℃水温下的游泳训练能使衰老时线粒体中的ＭＤＡ含量减少，膜流动性增加。适当的应激可能通过减轻自由基的损害而增加衰老小鼠海马线粒体膜的流动性。
The purpose of this study is to investigate the effect of
proper stress on the fluidity of mitochondrial membrane in hippocampus of aging mice.The
experiments were carried out on aging Kunming mice induced by Dglactose under swimming
stress for six weeks. Using DPH as fluorescent probe,the viscous coefficient() of mitochondrial
membrane was determined,and the content of MDA in mitochondria of hippocampus was
measured by relative agent box.We found that the fluidity of mitochondrial membrane in
hippocampus of aging mice reduced,and the content of MDA in mitochondria increased. But the
swimming stress at 18 water temperature for 15 min can enhance the fluidity of mitochondrial
membrane and reduce the content of MDA in mitochondria of aging mice.The results suggest
that the proper stress could be enhance the fluidity of the mitochondrial membrane of
hippocampus in aging mice by alleviating the damage of free radicals.
基金
江苏省教委自然科学基金
