链脲佐菌素加高糖高脂饮食复制大鼠2型糖尿病模型

Rat Model of Type 2 Diabetes Induced by Streptozotocin and HighCarbonhydrate-Fat Diet

目的链脲佐菌素加高糖高脂饮食诱导大鼠2型糖尿病模型的建立。方法Wistar大鼠分别高糖高脂饲料喂养4、6、8周后．采血检测空腹血糖及血清胰岛素，按30m/kg体重剂量一次性腹腔内注射链脲佐菌素，7d后再次采血检测糖尿病鼠空腹血糖及血清胰岛素。结果与对照组比较，高糖高脂喂养大鼠血清胰岛素明显上升（P〈0．05），但血糖无变化，糖尿病鼠血糖及血清胰岛素均皿著高于对照组（P〈0．05）。结论高脂高糖饲料喂养6周后，小剂量（30mg／kg）注射链脲佐菌素（STZ）诱导2型糖尿病大鼠模型，所建札的2型糖脲病模型与文献报道的方法相比成模率较高，死亡率较少。

Objective To establish a rat model of type 2 diabetes induced by streptozotocin and high- carbonhydrate-fat diet. Methods Healthy adult wistar rats were raised for four, six, eight weeks by high- carbonhydrate-fat feeds.The blood was collected to determine the blood glucose and plasma insulin levels.The rats of experimental group received intraperitoneal injection of streptozotocin once in a dose of 30mg/kg · b.w.Blood samples were taken to detect blood glucose and serum insulin revels in the diabetic rats at the one week. Results Serum insulin level in the high-carbonhydrate-fat feeds group was significantly increased as compared with that of control group（P〈0.05）,but the blood glucose level was not changed.The blood glucose and serum insulin levels in the diabetic rats were both significantly increased as compared with those in the control group（P〈0.05）. Conclusion A type 2 diabetes animal model with apparent hyperinsulinism and abnormal glucose tolerance has been successfully established in rats raised by high-carbonhydrate-fat feeds six week and combined with an intraperitoneal injection of streptozotocin once in a low dose.Compared with literatures,our method has high model forming rate and low mortality rate.