摘要
目的：观察实验性自身免疫性脑脊髓炎小鼠中枢及外周CD4^＋CD25^＋调节性T细胞（CD4^＋CD25^＋Treg），CD8^＋CD28^-调节性T细胞（CD8^＋CD28^-Treg）表达的变化情况，并探讨相关的细胞免疫学机制。方法：雌性C57BL／6小鼠随机分为未使用髓鞘少突胶质细胞糖蛋白35—55（MEVGWYR-SPFSRVVHLYRNGK）（MOG35-55）免疫的对照组和使用MOG35-55免疫诱导的EAE小鼠模型组，采用临床症状评分记录小鼠行为学变化、HE染色观察CNS炎症细胞浸润及病理改变，使用流式细胞术（FCM）检测小鼠中枢及外周CD8^＋CD28^-Treg，CD4^＋CD25^＋Treg细胞表达水平。结果：MOG35-55诱导的EAE模型组动物出现典型的EAE临床行为学及病理学表现，FCM检测EAE模型组小鼠脾细胞CD4^＋CD25^＋Treg较对照组升高但无统计学差异，CD8^＋CD28^-Treg表达水平明显低于对照组（P〈0．01），EAE模型组中枢有CD4^＋CD25^＋Treg，CD8^＋CD28^-Treg淋巴细胞的浸润，且CD4^＋CD25^＋Treg，CD8^＋CD28^-Treg在中枢的表达均高于外周，对照组中枢神经系统未检测到淋巴细胞浸润。结论：CD4^＋CD25^＋Treg，CD8^＋CD28^-Treg均参与调控EAE的病理过程，CD4^＋CD25^＋Treg，CD8^＋CD28^-Treg在EAE小鼠中枢及外周分布及变化的不同，提示其进人中枢神经系统（CNS）并参与调节中枢局部炎症。
AIM： To observe the change of periphery and centra CD4^＋ CD25~ Treg, CD8^＋ CD28^- Treg of MOG35-55 induced EAE disease in mouse, and to explore the potential mechanism of cellular immunity in the process of EAE. METHODS： MOG35-55 were used to establish EAE model on femina C57BL/6 mice. The behavioral changes and the histological scores were recorded. The changes of CD4^＋ CD25^＋ Treg, CD8^＋ CD28^- Treg on periphery and centra lymphocyte in spleen , brain were analyzed by flow cytometry. RESULTS： MOG35-55-induced EAE group Showed the typical clinical behavior and pathological manifestations, CD4^＋ CD25^＋ Treg, CD8^＋ CD28^- Treg lymphocytes were detected in the bran and spinal cord of EAE group mice, but they were not detected in the brain of control group. CD8^＋ CD28^-Treg in the spleen of EAE group were lower than those in control group （ P 〈 0.01 ）. CD4^＋ CD25^＋ Treg lymphocytes were slight higher than the control group. CONCLUSION： CD4^＋ CD25^＋ Treg, CD8^＋ CD28^- Treg lymphocytes all play important roles in the pathogenesy of EAE. The distribution of CD4^＋ CD25^＋ Treg, CD8^＋ CD28^- Treg in the CNS and peripheral of EAE is different, suggesting that their entry into the CNS and regulate of local inflammation.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2010年第8期746-749,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
浙江省自然科学基金资助项目(Y207494)
