高效毛细管电泳同时分离多种酚类物质

Simultaneous Separation of Polyphenols by High Performance Capillary Electrophoresis

建立了高效毛细管电泳同时分离测定芦丁、槲皮素、绿原酸、咖啡酸、没食子酸和原儿茶酸6种酚类物质的分析方法。考察了缓冲液种类、离子浓度和pH、分离电压、运行温度等电泳参数,确立了最佳的电泳条件：缓冲液为20 mmol/L磷酸二氢钠-20 mmol/L硼砂,pH7.5,分离电压为20 kV,运行温度为30℃,紫外检测波长214 nm。结果表明,6种物质在12 m in内得到完全分离,且各组分质量浓度与峰面积呈良好的线性关系,R2为0.998 7~0.987 4。方法精密度试验中,迁移时间的RSD为0.280%~0.425%,峰面积的RDS为5.228%~8.506%,回收率为85.11%~101.98%。该法快速、简便、准确,具有较高的灵敏度。

A high performance capillary electrophoresis method has been developed for the simultaneous separation and determination of six phenolics of rutin、quercetin、chlorogenic acid、caffeic acid、gallic acid and protocatechuic acid.The effects of several factors such as the pH and concentration of running buffer,the separation voltage and the runnion tamperation on HPCE were investigated.Under optimum condition,the running buffer consisted of 20 mmol/LNaH2PO4-20 mmol/L sodium borate,pH 7.5,separation voltageat 20 kV,running temperation at 30 ℃,the UV detection wavelength was 214 nm.The results showed that the six analytes can be well separated within 12.0 min,each one had a good linear relationship（R2=0.998 7～0.987 4）.The RSD of migration time was 0.280%～0.425% and of the peak area was 5.228%～8.506%.The average recovery percentage was 85.11%～101.98%.The method is rapid,simple and accurate.