牛主动脉蛋白聚糖对培养的人主动脉平滑肌细胞生长的影响

Effects of Bovine Aortic Proteoglycans on the Growth of Cultured Human Aortic Smooth Muscle Cells

为探讨蛋白聚糖在动脉粥样硬化发生发展中的作用，观察牛主动脉硫酸肝素蛋白聚糖、硫酸软骨素蛋白聚糖、硫酸皮肤素－硫酸软骨素蛋白聚糖和三种蛋白聚糖的混合物对培养的人主动脉平滑肌细胞增殖的影响。用细胞计数计算硫酸肝素蛋白聚糖（１．５～７．０ｍｇ／Ｌ）对培养的人主动脉平滑肌细胞增殖的抑制率分别为０、５５％和７６％；硫酸软骨素蛋白聚糖（１５．０～６０．０ｍｇ／Ｌ）的抑制率分别为２３％、３４％和６５％；硫酸皮肤素－硫酸软骨素蛋白聚糖（１５．０～６０．０ｍｇ／Ｌ）的抑制率分别为１９％、３７％和５９％；三种蛋白聚糖混合物（１０．５～４２．０ｍｇ／Ｌ）的抑制率分别为３３％、５５％和６５％。用氚标胸腺嘧啶脱氧核苷掺入法计算硫酸肝素蛋白聚糖（１．５～７．０ｍｇ／Ｌ）对培养的人主动脉平滑肌细胞增殖的抑制率分别为０、６８％和９１％；硫酸软骨素蛋白聚糖（１５．０～６０．０ｍｇ／Ｌ）的抑制率分别为４８％、６６％和８０％；硫酸皮肤素－硫酸软骨素蛋白聚糖（１５．０～６０．０ｍｇ／Ｌ）的抑制率分别为４９％、６３％和７２％；三种蛋白聚糖混合物（１０．５～４２．０ｍｇ／Ｌ）的抑制率分别为６５％、７９％和９１％。结果表明，硫酸乙酰肝素蛋白聚糖、硫酸软?

Aim The effect of bovine aortic heparan sulfate proteoglycan (HSPG), chondroitin sulfate proteoglycan (CSPG), dermatan chondroitin sulfate proteoglycan (DSCSPG) and mixed PG on the growth of cultured human aortic smooth muscle cells (hASMC) was studied in this article. Methods PGs were isolated by dissociative extraction, ion exchange chromatography and gel filtration from bovine aortic intima media and their effects on the growth of cultured hASMC were studied by cell count and 3 H TdR incorporation. Cultured hASMC were firstly inhibited at G0/G1 by DMEM containing 0.2% human serum (HS) and 0.2% fetal calf serum (FCS), then incubated with DMEM containing 5% HS and 5% FCS and different concentrations of PGs (PGs were presented as hexuronic acid) together with controls of DMEM without PG for 48 h. Results The inhibiting percentages of proliferation (based on cell count or 3 H TdR incorporation) of hASMC with HSPG (1.5～7.0 mg/L)were 0, 55% and 76%(0, 68% and 91%); with CSPG (15.0～60.0 mg/L) were 23%, 34% and 65% (48%, 66% and 80%); with DSCSPG (15.0～60.0 mg/L) were 19%, 37% and 59% (49%, 63% and 72%) and with mixed PGs (10.7～42.6 mg/L) were 33%, 55% and 65% (65%, 79% and 91%), respectively. Conclusion The that bovine aortic HSPG, CSPG, DSCSPG could inhibit the proliferation of hASMC and the inhibiting rate(%) were associated with the PG concentrations.