甲基莲心碱在体外对抗脂蛋白的氧化

The Effects of Neferine on Copper Mediated Oxidative Modified Lipoproteins

为探讨甲基莲心碱能否对抗脂蛋白的氧化，本文检测了甲基莲心碱对于Ｃｕ２＋介导低密度脂蛋白和极低密度脂蛋白氧化修饰的效应。结果发现，甲基莲心碱能抑制低密度脂蛋白和极低密度脂蛋白的硫代巴比妥酸反应物质的生成，还能抑制Ｃｕ２＋介导的极低密度脂蛋白电泳迁移率的增加。用甲基莲心碱保护的低密度和极低密度脂蛋白引起巨噬细胞内胆固醇和甘油三酯的堆积均低于相应的未保护脂蛋白。结果提示，甲基莲心碱能够在体外对抗Ｃｕ２＋介导低密度和极低密度脂蛋白的氧化修饰，而且由此抑制了巨噬细胞对脂蛋白的摄取。

Aim To study the effect of neferine (Nef) on copper mediated low density lipoprotein (LDL) and very low density lipoprotein (VLDL) oxidative modification. Methods Lipoproteins (LDL or VLDL) was isolated by ultracenfugation from normal human plasma. The extant of lipid peroxidation was measured in terms of thiobarbituric acid reactive substances (TBARS) express as malondiadelhyde (MDA) equivalents. The electrophoretic mobility of lipoproteins was determined on an agrose gel eletrophoresis. Mouse peritoneal macrophages (MP) was obtained by the method of Goldstein. Results Neferine inhibited the generation of TBARS in LDL and VLDL. Nef could decrease the electrophoretic mobility of VLDL, but it couldn't do this to LDL. The oxidized low density lipoproteins (ox LDL) and oxidized very low density lipoproteins (ox VLDL) was more efficient than Cu 2+ + Nef + LDL and Cu 2+ + Nef+ VLDL in stimulating lipid accumulation in MP. Conclusions Neferine can inhibit copper mediated LDL or VLDL oxidation and by which inhibit lipid accumulation in MP.