血单个核白细胞中CD36抗原基因表达的测定方法及其意义

The Determination and Significance of CD36 Antigen Gene Expression in Periphery Blood Mononuclear Leukocytes

为建立外周血单个核白细胞ＣＤ３６抗原基因表达的测定方法，本文采用反转录聚合酶链反应技术定量测定ＣＤ３６抗原基因的表达，用β－肌动蛋白作为内标物，用凝胶扫描技术进行定量。结果发现，反转录聚合酶链反应后电泳发现ＣＤ３６抗原ｃＤＮＡ片段为３９０碱基对，β－肌动蛋白ｃＤＮＡ片段为５４０碱基对，以ＣＤ３６抗原／β－肌动蛋白表示ＣＤ３６抗原基因表达量是一个稳定的方法，颈动脉粥样硬化病人ＣＤ３６抗原基因表达增加。结果提示，反转录聚合酶链反应是测定ＣＤ３６抗原基因表达的理想方法，可以用于动脉粥样硬化的研究。

Aim A method to determining CD36 antigen gene expression in periphery blood mononuclear leukocytes (PBMNL)is established. Methods The expression of CD36 antigen gene was measured by reverse transcription polymerase chain reaction (RT PCR)with β actin as the internal standard. RT PCR products were quantified by gel scanning. Results The gel electrophoresis revealed that the molecular weight of CD36 antigen RT PCR products was 390bp, that of β actin was 540bp. It is a stable method that CD36 antigen gene expression was expressed by CD36 antigen/β actin . The gene expression was significantly enhanced in the patients with carotid atherosclerosis. Conclusion RT PCR is an ideal method to determine expression of CD36 antigen gene, which might be used in the study of atherosclerosis.