恶性肿瘤p53基因异常、nm23表达与化疗敏感性关系

ASSOCIATION BETWEEN p53 GENE ABNORMALITY、nm23 EXPRESSION AND CHEMOSENSITIVITY IN MALIGNANT TUMORS

目的探讨恶性肿瘤ｐ５３基因异常、ｎｍ２３表达与抗癌药耐药性关系。方法用ＭＴＴ法测定１７种常用抗癌药对７４例恶性肿瘤的体外敏感性，并用聚合酶反应———单链构象多态分析技术和免疫组织化学染色法检测其ｐ５３基因异常、ｎｍ２３表达情况。结果与ｐ５３基因正常、ｎｍ２３（＋）组比较，其余三组抗癌药抑制率较小，其中ｐ５３基因正常、ｎｍ２３（－）组抗癌药抑制率不存在显著差异；ｐ５３基因异常、ｎｍ２３（＋）组对两种抗癌药抑制率存在显著差异；ｐ５３基因异常、ｎｍ２３（－）组对７种抗癌药抑制率存在显著差异。结论当恶性肿瘤中存在ｐ５３基因异常或ｎｍ２３表达（－）时，抗癌药耐药性增加。

PURPOSE In order to find out the relationship between p53 gene abnormality、nm23 expression and antitumor drug′s resistance in malignant tumors,and to explore the mechanism of antitumor drug resistance.METHODS To test the in vitro chemosensitivity of 74 malignant tumors for 17 antitumor drugs using MTT asay,and to detect p53 gene abnormality、nm23 expression using polymerase chain reaction(PCR) single strand conformation polymorphism and immunohistochemical method.RESULTS Compared with p53 gene normal、nm23(+) group,the inhibition rates of the antitumor drugs were lower in the other three groups.No significant difference was found between the p53 gene normal、nm23(+) group and p53 gene normal、nm23(-) group. There existed significant differences between p53 gene normal、nm23(+) group and p53 gene abnormal、nm23(+) group in 2 antitumor drugs,it was also present between p53 gene normal、nm23(+) group and p53 gene abnormal、nm23(-) group in 7 antitumor drugs.CONCLUSION When p53 gene abnormality or nm23(-) was present in malignant tumor,the antiumor drug resistance increased.The influence of p53 gene abnormality on the antitumor drug resistance was higer than low expression nm23.When p53 gene abnormality and nm23(-) coexisted in malignant tumors,the antitumor drug resistance was further increased.