非诺贝特对大鼠勃起功能和睾酮水平的影响

Effects of fenofibrate on rat erectile function and testosterone level

目的:观察非诺贝特对大鼠勃起功能和睾酮水平的影响,探讨非诺贝特致勃起功能障碍(ED)的原因。方法:30只雌性SD大鼠去势2周后分别在交配实验前48h和4h皮下注射苯甲酸雌二醇20μg和黄体酮500μg。将30只雄性大鼠随机分为2组:非诺贝特组和对照组,每组15只。非诺贝特组大鼠用非诺贝特31.5mg/kg溶于蒸馏水中(总体积4mL)灌胃,1次/d,连续14d;对照组大鼠用同等容积蒸馏水灌胃,1次/d,连续14d。在停药后第1天行交配实验,观察记录30min内2组大鼠的骑跨次数(MF)、插入次数(IF)、骑跨潜伏期(ML)和射精潜伏期(EL),并用放射免疫法检测血清和阴茎组织中睾酮水平。结果:非诺贝特组大鼠的MF和IF分别为(9.45±1.65)和(4.15±1.38)次/min、ML和EL分别为(14.1±7.0)和(5.3±5.9)min,对照组大鼠上述指标分别为(31.23±3.85)和(12.80±3.02)次/min、(6.2±4.3)和(16.2±6.0)min,差异有统计学意义(均P<0.05);大鼠血清和阴茎组织的睾酮水平非诺贝特组分别为(51.26±34.15)和(62.57±46.37)nmol/L,对照组分别为(153.72±83.93)和(164.89±94.02)nmol/L,差异有统计学意义(均P<0.05)。结论:非诺贝特能降低大鼠的性功能和睾酮水平。非诺贝特引致睾酮水平降低可能是勃起功能障碍的主要原因之一。

Objective： To observe the effects of fenofibrate on rat erectile function and testosterone level in order to explore the reasons of fenofibrate-induced erectile dysfunction（ ED） . Methods： Thirty female SD rats were unsexed and,2 weeks later,received SC injection of estradiol benzoate 20 μg and progesterone 500 μg 48 hours and 4 hours before mating experiment,respectively. Thirty male SD rats were randomly divided into two groups： the fenofibrate group and the control group. Each group comprised 15 rats. The rats in the fenofibrate group were gavaged with fenofibrate （ 31. 5mg/kg） dissolved in distilled water （ total volume 4mL） once daily for 14 days. The rats in the control group were gavaged with same volume of distilled water once daily for 14 days. The mating experiment was performed on the first day after medication withdrawl. The mount frequency （ MF） ,intromission frequency （ IF） ,mount latency （ ML） and ejaculation latency （ EL） of the rats in the two groups within 30 minutes were observed and recorded. The levels of testosterone in serum and penile tissue were measured using radioimmunoassay. Results： The MF and IF of rats in the fenofibrate group were （ 9. 45 ± 1. 65） ,（ 4. 15 ± 1. 38） times/min,ML and EL were （ 14. 0 ± 7. 0） ,（ 5. 3 ± 5. 9） minutes,respectively. The MF and IF of rats in the control group were （ 31. 23 ± 3. 85） ,（ 12. 8 ± 3. 02） times/min,ML and EL were （ 6. 2 ± 4. 3） ,（ 16. 2 ± 6. 0） minutes,respectively. The differences were statistically significant （ all P〈0. 05） . The levels of the testosterone in serum and penile tissue were （ 51. 26 ±34. 15） ,（ 62. 57 ±46. 37） nmol/L in the fenofibrate group,and （ 153. 72 ±83. 98） ,（ 164. 89 ±94. 02） nmol/L in the control group,respectively. The differences were statistically significant （ all P〈0. 05） . Conclusion： Fenofibrate can decrease rats’ erectile function and testosterone level. The decreasing testosterone level induced by fenofibrate might be one of the main reasons of ED.