归芪颗粒治疗猪血清所致大鼠肝纤维化的实验研究

Experimental study of guiqi granule on curing porcine serum-induced hepatic fibrosis in rats

目的研究中药归芪颗粒对猪血清所致肝纤维化模型大鼠的治疗作用。方法腹腔注射给予大鼠猪血清0.5 ml/只,2次/周,连续8周,建立大鼠肝纤维化模型。模型大鼠随机分为模型对照组、阳性药对照组及归芪颗粒高、中、低剂量组,另设正常大鼠对照组,第8周起按22.4、11.2和5.6 g.kg-1的剂量灌胃给予归芪颗粒进行治疗,连续8周,阳性对照组灌胃给予扶正化瘀胶囊0.825 g.kg-1,正常及模型对照组灌胃等容积的消毒饮用水。末次给药后次日取血,观察各组大鼠血清学指标(ALT、AST、T-BIL、ALP、TP、ALB、GLB、HA、CIV和PⅢNP)的改变及肝组织中羟辅氨酸(Hyp)的变化,并解剖大鼠取肝脏做病理组织学检查,观察肝脏纤维化程度。结果复方归芪颗粒能明显降低血清中谷草转氨酶(AST)、碱性磷酸酶(ALP)、透明质酸(HA)及肝组织中羟辅氨酸(Hyp)的水平,明显减轻肝脏细胞的病理损伤。结论复方归芪颗粒免疫损伤所致对大鼠肝纤维化症状有明显的改善作用。

Aim To study the effects of Guiqi granule on curing hepatic fibrosis model rats which induced by Porcine serum. Methods Fibrosis model was induced in rats by intraperitoneal injection with Porcine serum biweekly at dose of 0.5ml/time for 13 weeks. Then the model rats were randomly divided into model control group,positive control group and 3 Guiqi granule groups with high,middle and low dose. Another normal control group was set up with normal rats. The rats of Guiqi granule groups were treated with Guiqi granule per oral from the 8th week for 8 weeks with the dosages of 22. 4,11.2 and 5.6 g · kg^-1 （ calculated by raw materials）. The rats of positive control group were treated by FuZheng-Hua-Yu Capsule at dose 0. 825 g · kg^-1 per oral for same time. The normal control group and the model control group were given sterile drinking water at the same volume and same time of the other groups. At the second day of the last orally treated,the blood samples were taken to determine the changes of following serological parameters （ALT, AST,T-BIL, ALP ,TP, ALB, GLB, HA, CIV and PⅢNP） and the concentration of Hyp in liver tissue. Then liver tissue samples were taken to pathological sections for observing the changes of hepatic fibrosis. Results Guiqi granule could obviously decrease the levels of AST,ALP and HA in rats serum with experimental hepatic fibrosis model. It could evidently decrease the concentration of Hyp in liver tissue too,and could obviously relieve the pathological damage of liver tissues. Conclusion Guiqi granule could evidently improve the symptoms of hepatic fibrosis in rat immunity injury model.