TaqMan MGB双探针法和通用模板PCR法检测慢性乙型肝炎病毒YMDD变异的比较

Comparative studies on detection of YMDD mutation in hepatitis B virus by TaqMan MGB-PCR and UT-PCR

目的对检测HBV YMDD变异的TaqManMGB双探针法和通用模板PCR法做方法学比较,探讨其临床实用性。方法分别以TaqManMGB双探针和通用模板PCR法,对拉米夫定治疗过程中血清出现HBV DNA由阴转阳的87例慢性乙型肝炎患者,进行HBV YMDD变异检测,并以测序结果为标准,比较它们的灵敏度和特异性。结果TaqMan探针法和通用模板PCR法检测灵敏度分别为100%和93.02%,特异性均为100%,与测序法的符合率为98.9%和96.6%。两种方法学敏感度分别为5.0×105copies.L-1和1.0×106copies.L-1。结论TaqMan探针法检测HBV YMDD变异灵敏度高、特异性好,是临床检测HBVYMDD的较好方法。

Aim To compare the sensitivity and specificity of TaqMan MGB-PCR and UT-PCR methods used to detect YMDD mutation in hepatitis B virus （HBV）. Methods The sensitivity and specificity of TaqMan MGB-PCR and UT-PCR were compared with the results of DNA sequence through detection of HBV YMDD mutation in 87 serums from chronic hepatitis B patients receiving lamivudine monotherapy at the time of viral breakthrough. Results The sensitivities of HBV YMDD mutation detected by TaqMan MGB-PCR and UT-PCR were 100% and 93.02%. There were no differences between them（ P 〉 0.05 ）. Their speeificities were both 100%. Their rates of consistency with sequence method were 98.9% and 96.6%. Conclusion TaqMan MGB-PCR is a very good approach for detection of HBV YMDD mutations due to the higher sensitivity and specificity.