1Ahuja, M.R.Protoplast research in woody plant [J]. Silvae Genetica, 1984,33(1) :32-37.
2Russell J.A. and McCown R. H.. Culture and regeneration of Populus leaf protoplasts isolation from non-- seeding tissue [J ] . plant science, 1986,46L, 133-142.
6Santos A V P, Qutka D E, Cocking E C, et al. Organogenesis and somatic embryogenesis in tissue derived from leaf protoplasts and leaf explants of Medieago sativa [J].Zeitschrift für Pflanzenphysiol,1980,99(3):261-270.
7王海波.南方紫花苜蓿原生质体培养及再生体系的研究.重庆:西南大学,2007.
8George W Bates, John J Gaynor, Narpat S Shekhawat. Fusion of plant protoplasts by electric fields[J].Plant Physiology,1983,72(4):1110-1113.