三氯化铝比色法测定中草药雀儿舌头中总黄酮研究

AlCl_3 Colorimetry for Determination of Total Flavonoids Leptopus Chinensis Chinese Herbal Medicine

三氯化铝比色法的基本原理是利用芦丁与AlCl3发生显色反应,生成黄色络合物,在421 nm左右有较强吸收能力.而大多数黄酮类中药材多含有黄酮苷,常见的黄酮苷在421 nm波长处有较强吸收,而非黄酮类物质在此处无吸收.该方法实验条件容易控制,操作简单,平均回收率为102.52%,表明回收率的重现性好;而其相对标准偏差RSD为2.04%,表明方法的精密度好.因此以芦丁作为对照,可作为测定黄酮类化合物的常用方法.

The basic principles of aluminum chloride colorimetric method is the use of rutin and AlCl3 color reaction occurs to produce a yellow complex,which has strong absorption around 421 nm.Most of Chinese herbal medicines contain flavonoids glycosides and common flavonoid glycosides in the 421 nm wavelength has strong absorption,rather than the flavonoids here without absorption.This method is easy to control experimental conditions,simple operation,the average recovery was 102.52%,indicating good reproducibility and recovery rates;while the relative standard deviation RSD = 2.04%,indicating good precision approach.Therefore rutin can be a reference and used as a common measurement method of flavonoids.