Impacts of Moxibustion Therapy on Serum Corticosterone, Testosterone and Metabolism of Testicular Free Radicals in Rats with Fatigue

目的：从对血清皮质酮、睾酮水平和睾丸自由基代谢的影响，探讨艾灸疗法对运动性疲劳大鼠的作用机理。方法：SD大鼠随机分为对照组、模型组和治疗组。采用21d力竭游泳方式制作运动性疲劳模型。在造模成功后第21d观察大鼠力竭游泳时间，并在末次运动后24h取材。检测血清皮质酮、睾酮水平并计算睾酮／皮质酮比值，检测睾丸组织丙二醛（malondialdehvde，MDA）、谷胱甘肽（glutathione，GSH）、谷光甘肽过氧化酶（glutathioneperoxidase，GSH—Px）和超氧化物歧化酶（superoxidedismutase，SOD）的变化。结果：艾灸可以明显延长力竭游泳时间；明显降低血清皮质酮水平和增高睾酮／皮质酮比值；显著降低睾丸MDA含量，升高GSH、GSH—Px，但对SOD活性无显著影响。结论：艾灸疗法提高睾酮／皮质酮比值，降低睾丸自由基水平和提高抗自由基氧化酶活性，可能是艾灸疗法发挥抗疲劳作用的部分机制之一。

Objective： The study of the action mechanism of moxibustion therapy on sports fatigue in rats, in view of the expression of serum corticosterone, testosterone and testicula free radicals. Methods： Male SD rats were randomly divided into 3 groups： a control group, a model group and a treatment group. The rats were established to be in the sports fatigue model after exhaustive swimming for 21d. The time for the exhaustive swimming on the 21st day was recorded and the rats were killed in 24 h after the last swim. Samples were tested to determine the expression of serum corticosterone, serum testosterone, the ratio of testosterone and corticosterone （T/C）, testicular malondialdehyde （MDA）, glutathione （GSH）, glutathione peroxidase （GSH-Px） and superoxide dismutase （SOD）. Results： Moxibustion therapy prolonged the exhaustive swimming time, decreased serum corticosterone level, increased the T/C, reduced the MDA and improved the GSH and GSH-Px, but no significant effect was found on the SOD activity. Conclusion： The action mechanism of moxibustion therapy on release fatigue was supposed to be increasing the T/C, improving the testicular abilities of anti-oxygen free radicals and decreasing the free radical level.