川红拔瘤膏对小鼠S180瘤的抑制及其凋亡诱导作用的影响

Inhibitory effect of Chuanhong Baliu Paste on mouse S180 tumor and its apoptotic induction

目的:观察川红拔瘤膏对小鼠移植性肿瘤S180的抑制,探讨其诱导肿瘤细胞凋亡的作用。方法:建立小鼠荷瘤S180模型,随机分为5组,川红拔瘤膏按高、中、低3个剂量右腋下皮肤涂抹给药。模型组采用生理盐水灌胃给药,阳性组腹腔注射25mg/kg环磷酰胺。连续给药10d,观察瘤重、抑瘤率,DNA琼脂糖凝胶电泳和流式细胞仪器检测细胞凋亡与细胞周期变化。RT-PCR法检测凋亡基因Caspase-3的mRNA的表达。结果:与模型组比较,川红拔瘤膏高、中剂量能显著抑制小鼠S180的生长(P<0.05),可诱导肿瘤细胞凋亡,阻滞细胞周期在G0/G1期(P<0.05)。中、高剂量组均有Caspase-3表达的升高(P<0.05,P<0.01)。结论:川红拔瘤膏具有明显的抗肿瘤作用,作用机制可能是诱导凋亡,其诱导凋亡过程有Caspase-3的表达升高。

Objective：To observe the inhibition of Chuanhong Baliu Paste on S180 tumor and investigate its apoptotic induction. Methods：60 mice were divided into 5 groups randomly after inoculating S180 tumor. S180-bearing mice were smeared with different dosages of Chuanhong Baliu Paste respectively on skin of right armpit. Model group was administrated with normal sodium and positive group was injected intraperitoneally with Cyclophosphamide （25mg/kg） respectively. The growth inhibition was evaluated; apoptotic cells were detected by DNA agarose gelelectrophoresis and flow cytometry. The expression of Caspase-3 was determined by reverse transcription polymerase chain reaction method. Results：Compared with model group,Chuanhong Baliu Paste could signifi cantly inhibit the growth of S180 （P0.05）,could induce apoptosis of S180 tumor cells and arrest the cell cycle distribution at G0/G1 phase （P0.05）. The expression of Caspase-3 treated by middle and high dosages increased （P0.05,P0.01）. Conclusion：Chuanhong Baliu Paste could inhibit the tumor growth,the anti-tumor mechanism may be related to inducing the apoptosis of S180 cells and up-regulate the expression of Caspase-3.