补肾健脾中药对溴氰菊酯染毒大鼠妊娠结局的影响

Effect of invigorating the kidney and spleen Chinese material medica on deltamethrin intoxicated pregnant rats

目的:从防治溴氰菊酯(deltamethrin,DM)所致妊娠缺失方面研究补肾健脾复方助孕3号对DM生殖毒性的干预作用并初步阐明作用机制。方法:妊娠大鼠分5组,除空白对照组外,其中4组妊娠第1-15d予6.93mg/kgDM经口染毒,分别灌服生理盐水、成人等效、2倍、4倍剂量中药。第15d杀死半数母鼠,余下一半继续妊娠至分娩。观察流产率、活胎数、分娩情况,及雌二醇、孕激素水平。结果:①模型组流产率为30.77%,与其他组差异有显著性。中药高、中、低剂量组流产率分别为8.33、0、16.67%。②各组母鼠均在20-23d内分娩,分娩时未见异常。③妊娠中期(D15),模型组活胎率为(80.67±19.39)%,孕酮为(87.54±20.49)nmol/L,明显低于其他各组。E2水平各组间无明显差异。④分娩后,模型组P及E2水平均明显低于其他各组。结论:DM具有生殖毒性,可致妊娠丢失,其机制与影响母鼠内分泌功能有关,但不影响母鼠分娩。助孕3号方可干预DM毒性,降低流产率,可能与其内分泌调节作用有关。

Objective：To study the effect of invigorating the kidney and spleen complex prescription on deltamethrin intoxicated pregnant rats. Methods：Pregnant rats were divided into 5 groups. Except the blank group,other groups were orally fed 1/20LD50（6.93mg/kg）DM daily from day 1 to 15 when proved pregrancy. 4 Hours later,rats in model group were given physiological saline,other 3 groups were given high/ median /low dose of Zhuyun Formula. Half pregnant rats were killed on D15. The rest was kept until labour. The abortion rate,survival rate,delivery condition,the level of estradiol,progestogen were observed. Results：With respect to abortion rate,model group showed statistical signifi cance when compared with other groups. Model group also showed low progesterone level. With the treatment Zhuyun Ⅲ,it had desirable effect in decreasing abortion rate,and increasing the level of progesterone. The labour time of all groups was within the normal range. Conclusion：DM had toxicity on reproduction,it could lead to abortion which was related to endocrine function of pregnant rats. Zhuyun Ⅲ could regulate the above condition and relief DM toxicity.