β2-肾上腺素受体基因多态性及单倍体型与高血压病血瘀证的相关性分析

Relationship between polymorphisms of β2-adrenoceptor gene and hypertension with syndrome of blood stasis

目的:探讨β2-肾上腺素受体(β2-AR)基因-1023G/A、+252G/A多态性与高血压病血瘀证的关系。方法:收集高血压病血瘀证患者532例,高血压病非血瘀证298组,血压正常对照者512例,抽外周静脉血提取DNA,采用PCR-RFLP检测β2-AR基因-1023G/A、+252G/A多态性类型,用SHEsis软件构建单倍体数量。结果:χ2分割显示:重度血瘀组的β2-AR/-1023基因AA频率(14.92%)高于轻度血瘀组(6.95%)、非血瘀组(6.38%)、对照组(5.86%)(χ2=18.061,P<0.001)。多因素Logistic回归分析,以非血瘀组为参照系,调整诸危险混杂因素后,AA型患重度血瘀证的相对危险度(OR值)为3.667(95%CI:1.749-7.688,P=0.001);以G-G单倍体型(-1023G/A-+252G/A)为基线,A-A患血瘀证的OR值为1.818(95%CI:1.136-2.910,P=0.013),预测强度最大。结论:携带有β2-AR基因-1023AA型可能是重度血瘀证易患因素之一,A-A单倍体型可能易患血瘀证。

Objectives：To approach the relationship between the polymorphisms of beta2-adrenoceptor （β2-AR） gene 1023G/A （rs2053044）,＋252G/A （rs1042717） and essential hypertension （EH） with blood stasis syndrome （BSS）. Methods：The study cohort recruited 830 persons with EH and 512 normotensive controls,including 532 subjects of BSS （EH-BSS group） which made up of 331 subjects with low-grade BSS （I。-BSS） and 201 severe BSS subjects （II。-BSS）,298 subjects of no BSS （EH-non-BSS group） by TCM classifi cation. All the subjects were genotyped for the polymorphisms of β2-AR by PCR-RFLP,and the genetic variants frequencies were compared in subjects. The SHEsis program was employed to estimate the haplotype frequencies. Results：The patient genotype distribution was in Hardy-Weinberg equilibrium. Partitions of χ2 method showed that-1023AA was higher in II。-BSS group than in other groups （χ2=18.061,P0.001）. After adjusting for common risk factors of EH,logistic-regression indicated that the odds ratios （OR） of -1023AA （vs AG/GG） genotype for II。-BSS was 3.667 （95%CI：1.749-7.688,P=0.001） with the reference category of EH-non-BSS. The A-A（-1023-＋252） haplotype was more pronounced in patients with high-risk EH-BSS （adjusted OR=1.818,95%CI：1.136-2.910,P=0.013）. Conclusion：The-1023AA genotype may be some heritable susceptibility to disease of severe BSS,and A-A haplotype （-1023-＋252） was a susceptible factor to EH-BSS.