紫锥菊提取物的鼠伤寒沙门氏菌致突变试验研究

Experimental study of Ames test of Echinacea Herb P.E

目的:通过对紫锥菊提取物进行鼠伤寒沙门氏菌回复突变试验(Ames试验),评价该材料的潜在致突变性。方法:用紫锥菊提取物制备混悬液进行Ames试验,采用平板掺入法,计数TA97,TA98,TA100,TA102标准测试菌株在4个不同浓度下,计算于37℃培养48h后的回复突变菌落数。结果:无论在(+)S9和(-)S9的情况下,各剂量组均未引起测试菌株回变菌落数的明显增加,Ames试验结果为阴性。结论:紫锥菊提取物不会引起鼠伤寒门菌的回复突变数增加,说明紫锥菊提取物无致基因突变性。

Objective：To evaluate the potential mutagenicity of Echinacea Herb P.E via salmonella typhimurium Ames test. Methods：To test the Echinacea Herb P.E diffusion through Ames test. In the plate incorporation test of Salmonella Typhimurium,the average number of spontaneous revertants of TA97,TA98,TAl00,and TA102 at four concentrations was calculated after incubation at 37 ℃ for 48 hours. Results：Whether added S 9 or not,the number of induced revertants when compared to that of spontaneous revertants did not increase statistically significant. The result of Ames test was negative. Conclusion：Echinacea Herb P.E will not cause the increase of the back mutation in murine typhoid salmonella which shows that Echinacea Herb P.E will not cause gene mutation.