地塞米松对HaCaT细胞核因子-κB/IκB表达的影响

Effects of dexamethasone on the expression of NF-κB/IκB in human keratinocyte HaCaT cell line

目的:探讨地塞米松对角质形成细胞核因子(NF)-κB活性及其抑制蛋白(IκB)α表达的调节作用。方法:采用反转录-荧光实时定量PCR和蛋白印迹试验,观察经不同浓度及不同时间的地塞米松作用下,培养的人角质形成细胞HaCaT细胞的IκBα mRNA和蛋白质的表达;用蛋白印迹试验检测NF-κB核蛋白的表达情况。结果:地塞米松可诱导HaCaT细胞IκBα的表达而抑制NF-κB核转位,此作用呈浓度依赖性效应,在36 h时作用最明显。结论:糖皮质激素通过调控人角质形成细胞NF-κB/IκB信号通路,可抑制皮损局部的炎症反应。

Objective： To investigate effects of dexamethasone on regulation of NF-κB/IKB in human keratinocyte HaCaT cell line. Methods：Cultured HaCaT cells were treated by dexamethasone with different concentrations and different durations. The expressions of IκBα mRNA and protein were examined by reverse transcription-real time polymerase chain reaction （RT-PCR） and Western blot- ting. The expressions of NF-κB nucleoprotein were examined by Western blotting. Results：Dexamethasone could up-regulate the ex- pression of IκBα and meanwhile down-regulate the expression of NF-κB nucleoprotein in HaCaT ceils in a dose-dependent manner. The effects were most obvious with the treatment of dexamethasone for 36 h. Conclusion：Glucocorticoid might suppress inflammatory reaction by affecting NF-κB/IκB in skin lesions.