摘要
目的探讨不同溴代呋喃酮对聚氯乙烯(polyvinyl chloride,PVC)材料表面大肠杆菌生物膜形成的影响,为生物材料表面改性研究及临床生物材料植入感染的防治提供新思路。方法选用具有化学结构代表性的3种溴代呋喃酮,溴代呋喃酮1:3,4-二溴基-5-羟基-呋喃酮,溴代呋喃酮2:4-溴-5-(4-甲氧基苯基)-3-(甲氨基)-呋喃酮,溴代呋喃酮3:3,4-二溴基-5,5-二甲苯基-2(5H)-呋喃酮,分别对PVC材料片(1cm×1cm)进行表面涂层改性,将改性后的PVC材料片与大肠杆菌共同培养;将PVC材料片用75%乙醇浸泡5min后与大肠杆菌共同培养作为对照组。分别于培养6、12、18、24h,采用激光共聚焦显微镜动态观测PVC材料表面单位面积细菌群落数及细菌群落厚度,扫描电镜观察PVC材料表面细菌生物膜表面结构。结果激光共聚焦显微镜观测显示,各时间点溴代呋喃酮3组PVC材料表面单位面积细菌群落数以及细菌群落厚度均小于对照组,差异有统计学意义(P<0.05);而溴代呋喃酮1组和溴代呋喃酮2组与对照组比较差异均无统计学意义(P>0.05)。扫描电镜观察示,与对照组比较,溴代呋喃酮3组培养后6h PVC材料表面细菌群落附着数量较少;18h时对照组及溴代呋喃酮1组和溴代呋喃酮2组PVC材料表面细菌生物膜结构已初步形成,而溴代呋喃酮3组PVC材料表面无明显细菌生物膜结构形成。结论不同溴代呋喃酮对PVC材料表面大肠杆菌生物膜形成的影响不同,3,4-二溴基-5,5-二甲苯基-2(5H)-呋喃酮可抑制PVC材料表面单位面积大肠杆菌群落数和细菌群落厚度形成。
Objective To study the influence of brominated furanones on the biofilm formation of Escherichia coli on the polyvinyl chloride(PVC) material,and to provide new ideas for the research of surface modification of materials and clinical treatment of biomaterial centered infection.Methods Three brominated furanones with representative chemical structure were chosen and coated on the surface modification of PVC materials,respectively [furanone 1:3,4-dibromo-5-hydroxy-furanone;furanone 2:4-bromo-5-(4-methoxyphenyl)-3-(methylamino)-furanone;furanone 3:3,4-dibromo-5,5-bis(4-methylphenyl)-2(5H)-furanone].All the modificated PVC materials and Escherichia coli were co-cultivated.The PVC material soaked with 75% ethanol for 5 minutes and Escherichia coli were co-cultivated together as the control group.The thickness of bacterial community and bacterial community quantity in the unit area on PVC materials were measured by confocal laser scanning microscope(CLSM),and the surface structure of biofilm formation was observed by scanning electron microscope(SEM).Results The CLSM showed that the thickness of bacterial community and the bacterial community quantity in the unit area of PVC materials was significantly less(P 0.05) in furanone 3 group than in control group,but no significant difference(P 0.05) was found between furanone 1,furanone 2 groups and control group.SEM showed that the quantity of bacterial community in the unit area of PVC materials surface in furanone 3 group was fewer than that in control group at 6 hours;the biofilm structure on PVC materials surface formed at 18 hours in control group,furanone 1 group,and furanone 2 group,but there was no mature biofilm structure on PVC materials surface in furanone 3 group at 18 hours.Conclusion The impact of different brominated furanones on Escherichia coli biofilm formation on the surface of PVC materials is different,3,4-dibromo-5,5-bis(4-methylphenyl)-2(5H)-furanone can inhibit Escherichia coli biofilm formation on the surface of PVC material.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2010年第7期871-874,共4页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金资助项目(30872555)
云南省创新强省重点项目(2007C009Z)
云南省自然科学基金资助项目(2008ZC139M、2009CD184)~~
关键词
溴代呋喃酮
聚氯乙烯
细菌生物膜
大肠杆菌
Brominated furanone Polyvinyl chloride Bacterial biofilm Escherichia coli