HIV感染者、发病者及HAART治疗者NK细胞亚群变化研究

Alteration of NK cell subsets in HIV-infected Chinese

目的探讨HIV感染者、发病者和进行高效抗逆转录病毒疗法(HAART)的治疗者NK细胞亚群的变化情况。方法取新鲜外周全血,用荧光标记的单克隆抗体进行染色,经流式细胞仪检测分析HIV感染者、发病者和HAART治疗者NK细胞亚群的变化。结果 HIV感染者、发病者CD56dimCD16+NK细胞的百分比显著低于HIV抗体阴性健康对照;CD56-CD16+、CD56briCD16-/+NK细胞的百分比显著高于HIV抗体阴性健康对照;HAART治疗者CD56dimCD16+、CD56-CD16+和CD56briCD16-/+NK细胞亚群的百分比与HIV抗体阴性健康对照相比不再有显著差异。结论 HIV感染改变了NK细胞亚群的构成,HAART治疗后NK细胞亚群的比例可得到部分恢复。

To investigate changes of NK cell subsets in HIV-infected Chinese patients,PBMCs were collected from four groups representing different stages of HIV disease：asymptomatic chronically infected HIV patients,AIDS patients,HIV-positive patients receiving highly active antiretroviral therapy（HAART）,and HIV-negative controls.PBMCs were stained with fluorescent monoclonal antibodies for the NK cell subsets.Three distinct subpopulations（CD56-CD16＋,CD56dimCD16＋ and CD56briCD16-/＋） of NK cells were quantified by flow cytometer and compared among the groups.The percentage of CD56dimCD16＋ NK cells was decreased significantly,while the percentage of CD56-CD16＋ and CD56briCD16-/＋ NK cells were increased significantly in HIV group and AIDS group.The percentage of CD56dimCD16＋,CD56-CD16＋,and CD56briCD16-/＋ NK cells in HAART treatment group revealed no significant differences compared to any of the groups.Our results indicate that HIV infection changes the composition of NK cell subsets,and this change can be partially restored by HAART treatment.