Ⅱ型胶原诱导的小鼠关节炎模型IL-25表达水平的变化及意义

Expression of IL-25 in typeⅡ collagen-induced arthritis mouse model and its significance

目的以Ⅱ型胶原诱导的DBA/1小鼠关节炎模型为研究对象,动态观察其IL-25的表达水平,探讨IL-25与关节炎发生发展的关系及其可能的机制,为寻找新的类风湿性关节炎免疫干预新途径积累实验数据。方法采用RT-PCR法扩增目的基因,构建mIL-25标准质粒,QRT-PCR检测模型鼠脾脏及其关节病变局部IL-25的表达水平;应用ELISA技术检测模型鼠血清和脾细胞培养上清IL-25蛋白的含量。结果在Ⅱ型胶原诱导的小鼠关节炎的发病过程中,模型鼠脾细胞和关节滑膜组织IL-25的mRNA表达水平、均呈持续上升趋势,与对照小鼠相比差异显著(P<0.05)。这种IL-25蛋白和转录水平的表达,随着关节炎症程度的增加而升高。此外,模型鼠脾细胞对ConA的刺激表现出强IL-25表达性应答。结论 IL-25的表达水平与Ⅱ型胶原诱导的关节炎的发生发展密切相关。

With typeⅡ collagen-induced DBA/1 mouse model of arthritis,we studied the expression of IL-25 dynamically for approaching the relationship between IL-25 and the occurrence and development of arthritis as well as its possible mechanism,in order to accumulate the experimental data for new ways of immune intervention in rheumatoid arthritis.We amplified the mIL-25 gene by RT-PCR and constructed its standard plasmid.Then we detected the expression of mIL-25 in spleen and joint diseased region of the model by QRT-PCR,as well as the mIL-25 in serum and spleen cell culture supernatant by ELISA.The results showed that during the pathogenesis of type Ⅱcollagen-induced arthritis,the mRNA expression of IL-25 in the spleen cells and articular synovial tissue in the model mouse showed continuous upward trend,as compared with the control mice significantly different（P 0.05）.With the development of arthritis,the levels of IL-25 protein and transcription increased.In addition,the model mouse spleen cells showed strong response to ConA by expressing IL-25.The expression of IL-25 is closely related to the occurrence and development of typeⅡ collagen-induced arthritis.