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下调LINE-1编码蛋白ORF-1p对肺癌细胞A549生物学特征的影响 被引量:7

The Down-regulation of LINE-1 Encoded ORF-1p Impacts the Characters of A549 Cells
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摘要 目的:探索逆转座子LINE-1编码的ORF-1p在肺癌发病过程中的分子机制。方法:利用RNAi技术,在肺癌细胞A549中下调LINE-1编码蛋白ORF-1p,随后对下调后的A549细胞的生物学特征进行细胞增殖(MTT方法)、细胞周期(流式细胞技术)以及集落形成(软琼脂克隆形成试验)等分析,观察细胞生物学特征的改变。并利用报告基因,进一步对细胞周期相关蛋白进行分析。结果:在A549细胞中,下调ORF-1p后细胞的增殖能力明显下降(P<0.05),细胞周期出现S期明显阻滞(P<0.05),肿瘤细胞的集落形成能力明显减弱(P<0.05),p15/p21报告基因表达显示,两种蛋白被显著上调。结论:下调LINE-1基因编码蛋白ORF-1p能够抑制肺癌细胞的生长以及肿瘤的形成。 Objective:To investigate the mechanism of LINE-1 encoded ORF-1p in the tumorigenesis of lung cancer.Methods:The down-regulation of ORF-1p was conducted by RNAi approach and the features of transfected A549 cells were characterized by the approaches of MTT,flow cytometry and soft-agar anchored colony formation.Results:With the constructs of recombinant plasmids,LINE-1 encoded ORF-1p was down-regulated significantly in transfected A549 cells.The characters of transfected A549 cells were appeared to change as follows:Cell proliferation was significantly reduced,which was probably attributed to up-regulation of p21 and p15;cell cycle exhibited S phase arrest(P0.05);colony formation was significantly attenuated.Conclusion:The down-regulation of ORF-1p can attenuated the A549 characters significantly upon its tumor formation ability.
出处 《中国生物工程杂志》 CAS CSCD 北大核心 2010年第6期14-20,共7页 China Biotechnology
关键词 LINE-1 ORF-1p RNAI 肺癌 LINE-1 ORF-1p RNA interference Lung cancer
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  • 1Narita N, Nishio H, Kitoh Y, et al. Insertion of a 5truncated L1 element into the 3'end of exon 44 of the dystrophin gene resulted in skipping of the exon during splicing in a case of Duchenne muscular dystrophy. J Clin Invest, 1993, 91 : 1862-1867.
  • 2Bailey J A, Carrel L, Chakravarti A, et al. Molecular evidence for a relationship between LINE-1 elements and X chromosome inactivation: the Lyon repeat hypothesis. Proc Natl Acad Sci USA, 2000, 97:6634-6639.
  • 3Allen E, Horvath S, Tong F, et al. High concentrations of long interspersed nuclear element sequenee distinguish monoallelically expressed genes. Proc Natl Acad Sci USA, 2003, 100:9940-9945.
  • 4Burwinkel B, Kilimann M W. Unequal homologous recombination between LINE-1 elements as a mutational mechanism in human genetic disease. J Mol Biol, 1998, 277:513-517.
  • 5Liu L, Chen X H, Huang J, et al. NSSRI promotes neuronal differentiation of mouse embryonic carcinoma P19 cells. Neuroreport, 2004, 15:823-828.
  • 6Anthony V Furanol, David D Duvernell, Stephane Boissinot. L1 (LINE-1) retrotransposon diversity differs dramatically between mammals and fish. Trends in Genetics ,2004 ,20 ( 1 ) :9-14.
  • 7Xi Shi, Andrei Seluanov, Vera Gorbunova. Cell divisions are required for L1 retrotransposition. Molecular and Cellular Biology, 2007, 27(4 ) :1264-1270.
  • 8Roman-Gomez J, Jimenez-Velasco A, Agirre X, et al. Promoter hypomethylation of the LINE-1 retrotransposable elements activates sense/antisense transcription and marks the progression of chronic myeloid leukemia. Oncogene ,2005,24:7213-7223.
  • 9Goodier J L, Zhang L, Vetter M R, et al. LINE-10RF1 protein localizes in stress granules with other RNA-binding proteins, including components of RNA interference RNA-induced silencing complex. Mol Cell Biol,2007,27:6469-6483.
  • 10Wichai Pornthanakasem, Narisom Kongruttanachok, Chutipa Phuangphairoj. LINE-1 methylation status of endogenous DNA double-strand breaks.. Nucleic Acids Research, 2008, 6 ( 11 ) : 36673675.

同被引文献79

  • 1Fan Feng,Yin-Ying Lu,Fan Zhang,Xu-Dong Gao,Chuan-Fu Zhang,Alex Meredith,Zhong-Xian Xu,Yu-Tao Yang,Xiu-Juan Chang,Hong Wang,Jian-Hui Qu,Zhen Zeng,Jun-Lan Yang,Chun-Ping Wang,Yun-Feng Zhu,Jia-Jun Cui,Yong-Ping Yang.Long interspersed nuclear element ORF-1 protein promotes proliferation and resistance to chemotherapy in hepatocellular carcinoma[J].World Journal of Gastroenterology,2013,19(7):1068-1078. 被引量:8
  • 2Smit A F. The origin of interspersed repeats in the human genome. Curt Opin Genet Dev, 1996, 6 (6) : 743-748.
  • 3Daskalos A, Nikolaidis G, Xinarianos G. Hyqpomethylation of retrotransposabte elements correlates with genomic instability in non-small cell lung cancer, lnt J Cancer,2009,124( 1 ) :81-87.
  • 4Kirilyuk A, Tolstonog G V, Damert A. Functional endogenous LINE-1 retrotransposons are expressed and mobilized in rat chloroleukemia cells. Nucleic Acids Res, 2008, 36 ( 2 ) : 648- 665.
  • 5Bratthauer G L, Fanning T G. Active LINE-1 retrotransposons in human testicular cancer. Oncogene, 1992, 7 ( 3 ) : 507-510.
  • 6Martin S L. The L1-ORF1 protein encoded by LINE-1 :Structure anti function duringL1 retrotranspusition, j Biomed Biotechnol, 2006, 2006( 1 ) :45621.
  • 7Goodier J L, Ostertag E M, Engleka K A. A potential role for the nucleolus in LI retrotransposition. Hum Mol Genet, 2004, 13 (10) :1041-1048.
  • 8Crow M K. Long interspersed nuclear elements (LINE-1): Potential triggers of systemic autoimmune disease. Autoimmunity, 2010, 43( 1): 7-16.
  • 9Su Y, Davies S, Davis M. Expression of LINE-1 p40 protein in pediatric malignant germ cell tumors and its association with clinic opathological parameters: A report from the children' s oncology group. Cancer Lett, 2007,247 ( 2 ) : 204-212.
  • 10ROMAN-GOMEZ J,JIMENEZ-VELASCO A,AGIRRE X,et al.Promoter hypomethylation of the LINE-1 retrotransposable elements activates sense/antisense transcription and marks the progression of chronic myeloid leukemia[J].Oncogene,2005,24:7213-7223.

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