中药促生汤促小鼠宫颈成熟的实验研究

EXPERIMENTAL STUDY OF CUSHENBTANG ON PROMOTING THE CERVICAL RIPENING OF PREGNANT MICE

为研究中药促生汤促小鼠宫颈成熟的效果及其作用机制,以受孕昆明鼠作为实验对象,放免法检测小鼠血液E_2(雌二醇)、P(孕酮)水平及光镜下观察小鼠宫颈胶原纤维的变化。结果表明实验组与对照组相比:E_2水平明显升高(P<O.001),P水平明显下降(P<0.05),宫颈组织疏松、密度降低,胶原纤维破坏、断裂、排列紊乱,小鼠平均提前2d分娩,差别有显著性,而幼仔存活率无差别。说明中药促生汤有明显的促宫颈成熟的作用,其作用机制可能是通过改变E_2及P水平,影响宫颈胶原酶活性,加速宫颈胶原纤维断裂、降解、重新排列而实现的。

In order to sutdy the effects and the mechanism of CuShengTang on promoting the cervical ripening of the pregnant mice.The E2 and P levels of mice blood were tested by radioimmunoassay and the cervical collagen fibers of mice were examined under light microscope. Comparing with the control group: the experimental group delivered two days earlier than that of control, E 2. level was higher(p< 0.001) and P level was lower than that of control. (p< 0.05), the tissue of cervix was sparsed and the collagen fiber rearranged and broke . All these suggested CuShengTang clearly effect on promoting rhe cervical ripening of the pregant mice. Changing of E2、P level increased the activity of the cervical collagenase,leading to a rearrangment and break of the cervical collagen fibers.