济南假单胞菌制剂体外抑瘤作用及其机制的研究

Studies of Pseudomonas Jinanensis Agent on Tumor Inhibition Mechanism

为进一步探讨济南假单胞菌制剂（ＰＪＡ）抗瘤作用机制，将ＰＪＡ作用于人高转移肺癌细胞ＰＧ，采用ＭＴＴ、放免、流式细胞术、粘附实验、运动侵袭实验等多种手段进行了检测。显示ＰＪＡ有显著的抑瘤作用，并能影响ＰＧ细胞周期的时相分布，使Ｓ期减小。其对ＰＧ细胞内ｃＡＭＰ无明显影响，但能降低ｃＧＭＰ水平，提高ｃＡＭＰ／ｃＧＭＰ比值，同时降低ＰＧ细胞对胞外基质的粘附性及侵袭、运动能力，从而逆转肿瘤细胞的恶性表型。提示，ＰＪＡ作为一种新型抗癌药物具有较高的临床应用价值。

Pseudomonas Jinanensis Agent(PJA) is a kind of new anticancer drug. In order to further elucidate the anticancer mechanism of PJA, highly metastatic human lung cancer cells(PG) were treated with PJA in vitro . MTT assay, radioimmune assay, flow cytometry(FCM), and invasion assay were used. The results showed that PJA could inhibit the proliferation of PG, influenced the distribution of PG cells cycle and S phase was reduced. Although PJA didn′t change the level of cAMP in PG cells, it reduced the level of cGMP and increased the cAMP/cGMP ratio. On the other hand, PJA also decreased PG cells adhesive ratio to laminin substrate and decreased the ability of invasion or migration. These data demonstrated that PJA is a kind of effective anticancer drug.