蓝靛果忍冬茎段离体培养与植株再生

In vitro culture and plant regeneration from sprouting buds of Lonicera edulis Turcz

以蓝靛果忍冬的幼嫩茎段为外植体,采用正交设计及方差分析对影响蓝靛果忍冬植株离体培养与植株再生的因素进行了优化研究,培育出了蓝靛果忍冬的组培苗。结果表明:初代培养的适宜培养方案是MS+6-BA1.0mg/L+IBA0.2mg/L,芽分化率达到100%;继代增殖培养的适宜培养方案是MS+6-BA0.5mg/L+IBA0.3mg/L+KT1.5mg/L,增殖系数达到8.7;生根培养的适宜培养方案是1/4MS+IBA1.5mg/L,生根率达到96.7%。生根试管苗在驯化、移栽后成活率可达97.8%。6-BA、IBA和KT配合使用,可以增加蓝靛果忍冬继代培养的丛生芽数量。该试验培育的组培苗的各项指标已达到快速繁育苗木的要求,其技术措施可直接应用于规模化生产。

The factors affecting in vitro culture and plant regeneration of Lonicera edulis were optimized by orthogonal design and analysis of variance using sprouting buds as explants.Tissue cultured seedlings were obtained on culture media with different concentrations of hormones.The results show that the optimum compositions for primary culture,subculture and rooting culture are MS ＋ 6-BA 1.0 mg /L ＋ IBA 0.2 mg /L,MS ＋ 6-BA 0.5 mg /L ＋ IBA 0.3 mg /L ＋ KT 1.5 mg /L and 1 /4MS ＋ IBA 1.5 mg /L.The differentiation rate,proliferation coefficient and rooting rate on these media were up to 100% ,8.7 and 96.7% ,respectively.The survival rate of the rooted plantlets could reach 97.8% after acclimatization and transplantation.The combination of 6-BA,IBA and KT is effective in shoot proliferation of L.edulis in the subculture.This micropropagation technology could be applied in mass propagation.