针刺对急性手术创伤大鼠下丘脑促肾上腺皮质激素释放因子家族肽及其受体表达的影响

Effects of Electroacupuncture on the Expression of Corticotropin-releasing Factor (CRF) Family and CRF Receptor 1 in Traumatic Injury Rats

目的:通过观察针刺对急性手术创伤大鼠下丘脑促肾上腺皮质激素释放因子(CRF)家族肽及其受体表达的影响,探讨针刺调节急性手术创伤所导致的下丘脑-垂体-肾上腺(HPA)轴功能异常作用的神经内分泌机制。方法:雄性SD大鼠40只,随机分成正常组、创伤组、正常加电针组、创伤加电针组,每组10只。创伤动物在乙醚麻醉下行剖腹探查术。电针组动物电针"足三里""三阴交"30min。应用特异性放射免疫法检测各组动物血清促肾上腺皮质激素(ACTH)、皮质肌动蛋白(Cort)、促黄体生成素(LH)和肌钙蛋白(T)的水平,应用RT-PCR的方法观察各组动物下丘脑CRF家族肽和其受体mRNA的表达。结果:创伤组大鼠血清ACTH水平显著下降(P<0.05),创伤加电针组血清ACTH水平和创伤组相比显著升高(P<0.05);创伤组大鼠血清Cort水平较正常组显著上升(P<0.05),创伤加电针组血清Cort水平较创伤组显著降低(P<0.05);各组动物血清LH和T水平差异无统计学意义(P>0.05)。创伤组大鼠下丘脑CRF mRNA的表达和正常组相比降低(P<0.05),创伤加电针组大鼠下丘脑CRF mRNA的表达和创伤组相比升高(P<0.05);创伤组大鼠下丘脑Ucn1 mRNA的表达与正常组比较升高(P<0.05),创伤加电针组大鼠下丘脑Ucn1 mRNA的表达和创伤组相比下降(P<0.05);各组Ucn2、Ucn3和CRF受体1 mRNA的表达差异均无统计学意义(P>0.05)。结论:电针效应具有多环节、多靶点的特点,电针可能通过调整急性创伤大鼠下丘脑CRF和Ucn1 mRNA的表达,进而调整急性剖腹探查所致的大鼠HPA轴功能异常。

Objective To observe the effects of electroacupunture （EA） on the abnormal hypothalamus-pituitary-adrenal （HPA） axis and the expression of hypothalamic corticotropin-releasing factor （CRF） peptides family and CRF receptor （R）I mRNA in traumatized rats, so as to study its underlying mechanism in improving traumatic injury. Methods Forty male Sprague-Dawley rats were randomly divided into normal control group （n = 10）, normal ＋ EA group （n = 10）, surgical trauma （model） group （ n = 10）, and model ＋ EA group （ n = 10）. Traumatic injury model was established by performing an exploratory laparotomy in the rats under anesthesia. EA （2 Hz/15 Hz, ] -2 mA） was administered for 30 min via two stainless steel needles inserted in ＂Zusanli＂ （ST 36） and ＂Sanyinjiao＂ （SP 6）. Serum adrenocorticotropic hormone （ACTH） , corticosterone （Cort）, lu- teinizing hormone （LH） and testosterone （T） contents were detected by double-antibody radioimmnoassay. Hypothalamic ORF and CRF R1 mRNA expression was assayed by reversed transcription polymerase chain reaction （RT-PCR） technique. Results The serum ACTH concentration decreased significantly in model group （P〈0.05） compared with those in normal control and normal ＋EA groups, and was significantly higher in model4- EA group than that in model group （P〈0.05）. No significant difference was found between normal control and normal＋ EA groups in serum ACTH levels （P〈0.05）. The serum Cort level increased significantly in model group（ P〈0. 05） compared with those in normal control and normal4-EA groups, and was obviously lower in mo- del4- EA group than that in model group （P〈0.05）. While serum LH and T contents had no apparent changes in the four groups （P〉0.05）. The expression of hypothalamic CRF mRNA decreased significantly in model group （P〈0.05） compared with that in normal control group, but increased significantly in model＋ EA group （P〈0.05） compared with that in model group. However, no significant difference was found between normal control and normal 4- EA groups in the CRF mRNA expression level. The expression of hypothalamic Ucn 1 mRNA increased significantly in model group than that in normal control group （ P〈0.05）, and decreased considerably in model4- EA group than that in model group （P〈0.05）. There were no significant changes in the expression of hyp, thalamic Ucn 2 mRNA, Ucn 3 mRNA and CRF R｜ mRNA in the four groups （P〈0.05）. Conclusion EA of ＂Zusanli＂ （ST 36） and ＂Sanyinjiao＂（SP 6） can improve surgical trauma-induced HPA disorders, which is probably related to its effects in upregulating CRF mRNA expression and downregulating Ucn 1 mRNA expression in the hypothalamus.