血液制品丙肝病毒与艾滋病毒检测

Detection of HCV and HIV in fresh frozen plasma and plasma derivatives

目的了解１９９３～１９９５年度临床使用的新鲜冰冻血浆（ＦＦＰ）和血液制品中艾滋病毒（ＨＩＶ）、丙肝病毒（ＨＣＶ）的感染情况。方法用ＥＬＩＳＡ、ＷＢ和ＰＣＲ对１１０批ＦＦＰ、人血丙种球蛋白、人血白蛋白检测了抗ＨＣＶ、ＨＣＶＲＮＡ、抗ＨＩＶ（１＋２）、ＨＩＶ－１ＲＮＡ。结果所有血制品抗ＨＣＶ的平均阳性率为１９．１％，ＨＣＶＲＮＡ为１７．３％；１９９３～１９９４年度ＦＦＰ的抗ＨＣＶＨＣＶＲＮＡ阳性率比１９９５年度高（３０％、２６％和０．５％）；人血丙球的抗ＨＣＶ和ＨＣＶＲＮＡ阳性率明显高于人血白蛋白（３０％、２０％和０．５％）；８９批抗ＨＣＶ阴性血制品ＨＣＶＲＮＡ的阳性率为５．６％，所有血制品的抗ＨＩＶ（１＋２）和ＨＩＶ－１ＲＮＡ均阴性。结论１９９３～１９９５年度临床上所使用的血制品，特别是１９９３～１９９４年度的ＦＦＰ，有传播ＨＣＶ的危险，这与当时没有对所有供血者进行抗ＨＣＶ筛检有关。

OBJECTIVE To evaluate the risk of HCV and HIV infection from fresh frozen plasma (FFP) and plasma derivatives used in clinical during 1993～1995 were studied. METHODS 110 batches of FFP, human immunoglobulin and human serum albumin were detected for anti-HCV, HCV RNA, anti-HIV (1+2) 、HIV-1RNA by ELISA, western blot (WB) and PCR. RESULTS For all the plasma products, the average positive rate of anti-HCV was 19.1%, and HCV RNA 17.3%. The positive rates of anti-HCV and HCV RNA in FFP from 1993 to 1994 were higher than that in 1995 (30%, 26% and 0.5% respectively, P 1<0.01, P 2<0.05). The positive rates of anti-HCV and HCV RNA in human immunoglobulin were obviously higher than that in human serum albumin (30%, 20% and 0.5% respectively, P 1<0.01, P 2>0.05). For 89 batches of anti-HCV negative plasma products, the positive rate of HCV RNA was 5.6%. All plasma products detected were anti-HIV (1+2) and HIV-1RNA negative except one batch of human immunoglobulin was anti-HIV (1+2) positive in sereening test, but negative in WB confirmatory test. CONCLUSION THe results suggested that the plasma products used in clinical during 1993～1995, especially the FFP from 1993 to 1994, were not always safe. This risk was associated with that not all donors had been screened for anti-HCV then.