高效液相色谱法测定清感利咽颗粒中绿原酸、龙胆苦苷、葛根素的含量

To determination of chlorogenic acid,gentiopicrin and puerarin in Qingganliyan-Granules by HPLC

目的建立复方制剂清感利咽颗粒中绿原酸、龙胆苦苷、葛根素的含量测定方法。方法采用高效液相色谱法。采用Kromasil C18(4.6mm×250mm,5μm)色谱柱,流动相:甲醇-乙腈(1∶1)混合溶液(A)与0.4%磷酸溶液(B)梯度洗脱(0～30分钟,流动相A10%→16%;30～35分钟,流动相A16%);流速:1.0ml/min;检测波长:绿原酸327nm,龙胆苦苷278nm,葛根素250nm;柱温:室温。结果绿原酸、龙胆苦苷和葛根素分别在0.04～0.48μg(r=1),0.08～0.96μg(r=1);0.02～0.24μg(r=1)呈良好的线性关系。平均回收率分别为98.67%、97.72%、100.82%。结论本测定方法简便可行、重复性好,可用于本制剂中三种指标成分的质量控制。

Objective To establish a HPLC method for the determination of chlorogenic acid,gentiopicrin and puerarin in Qingganliyan-Granules. Methods A Kromasil C18 （4.6mm×250mm,5μm） column was adopted. The mobile phase was methanol and acetonitrile （ 1 ： 1 ） （ A ） -0. 4% phosphoric acid solution（B） in gradient mode（0 - 30min,10%→16% A;30 -35min,16% A）. The flow rate was 1.0 ml/ min. The temperature column was set at home temperature, and the wavelengths were set at 250 rim, 278 nm and 327 nm. Results The liner ranges of chlorogenic acid, gentiopicrin and puerarin were 0. 04 - 0. 48 μg（r = 1 ）, 0. 08-0. 96 tLg（ r = 1 ）, and 0. 02 - 0. 24μg（ r = 1 ） respectively. The average recovery was 98. 67% ,97.72%, 100. 82% respectively. Conclusion The method is simple, feasible and reproducible and can be used for the quality control of Qingganliyan-Granules.