B16F10黑素瘤细胞培养上清对同基因小鼠脾淋巴细胞增殖的抑制作用

Suppressed Proliferation of Syngenic Mouse Spleen Lymphocytes in Vitro by Supernatant from B16F10 Cell Culture

目的:研究B16F10黑素瘤细胞培养上清对同基因小鼠脾淋巴细胞的抑制作用。方法:制备B16F10黑素瘤细胞培养上清,作用于同基因小鼠脾淋巴细胞,通过PHA刺激和混合淋巴细胞反应,用MTT法检测淋巴细胞的增殖活性。结果:在B16F10黑素瘤细胞培养上清的作用下,PHA诱导的同基因小鼠脾淋巴细胞增殖率平均为63.00%,明显低于对照组,差异有显著性(t=16.447,p=0.000);混合淋巴细胞反应的淋巴细胞增殖率平均为61.82%,亦明显低于对照组,差异有显著性(t=8.097,P=0.000)。结论:B16F10黑素瘤细胞培养上清对同基因小鼠脾淋巴细胞的增殖具有抑制作用。

Objective：To study the suppression effect on proliferation of syngenic mouse spleen lymphocytes in vitro by supernatant from B16F10 cell culture.Method： The supernatant from B16F10 cell culture was prepared and used on syngenic mouse spleen lymphocytes.The proliferations induced by PHA or MLR were determined by MTT assay.Result： Treating with supernatant from B16F10 cell culture,the proliferations of mouse spleen lymphocytes induced by PHA and MLR were 63.00% and 61.82% respectively.Both were lower than that of their controls with statistically significant differences（t=16.447,p=0.000 and t=8.097,p=0.000 respectively）.Conclusion： The proliferation of syngenic mouse spleen lymphocytes was suppressed by supernatant from B16F10 cell culture.