采用S/D病毒灭活后凝血酶原复合物在犬非停滞模型上致血栓性的研究

Study on Thrombogenicity of Prothrombin Complex Concentrate Post Inactivation of Virus by S/D in Non stasis Canine Model

目的：进一步评价采用有机溶剂／表面活性剂（Ｓ／Ｄ）技术灭活病毒后新工艺凝血酶原复合物（ＰＣＣ）的潜在致血栓性。方法：采用犬非停滞模型进行实验研究，动态监测血小板数（即刻测定），Ｄ－二聚体、ＴＸＢ２、ＦⅤ∶Ｃ、ＦⅧ∶Ｃ、蛋白Ｃ和ＰＴ７项指标，并与未经病毒灭活处理的旧工艺ＰＣＣ进行比较。结果：新工艺ＰＣＣ组的上述指标动态变化所表明的潜在血栓倾向均明显低于旧工艺ＰＣＣ组。结论：经病毒灭活处理的新工艺ＰＣＣ不但病毒安全性已提高，其血栓形成的危险性也明显降低。

To evaluate further the potential thrombogenicity of the prothrombin complex concentrate(PCC) produced by new technological process with virus inactivation by S/D technique.Method:The experimental study was carried out in non stasis canine model,meanwhile the dynamic monitoring of the following seven indices was performed:platelete count(determinated immediately),D dimer,thromboxane B 2(TXB 2),factor V activity,factor Ⅷ activity,protein C activity and partial thromboplastin time(PT),and the new prothrombin complex concentrate was compared with the old one produced by technological process without virus inactivation treatment by S/D in respect of potential thrombogenicity.Results:The potential thrombogenic tendency of new prothrombin complex concentrate showed by dynamic changes of their indices mentioned above was obviously better than that of the old one.Conclusion:The new prothrombin complex concentrate not only has higher safety in terms of virus infection,but also has obviously lower risk of thrombogenicity.