摘要
目的:探讨孕激素拮抗剂米非司酮对患者子宫内膜癌细胞凋亡、周期及孕激素受体(PR)亚型的影响。方法:患者在米非司酮治疗前及治疗后分别留取标本60例,用流式细胞仪检测组织细胞凋亡率和周期时相的变化,用实时定量PCR检测PR-A mRNA和PR-B mRNA的表达情况。结果:患者经过米非司酮治疗后,癌细胞凋亡率和G0/G1比率升高,S期比率下降,RR-B mRNA表达显著下降,PR-B与PR-A的比例明显降低。结论:米非司酮下调PR-B的表达,使癌细胞阻滞于G1期,促进癌细胞凋亡,抑制子宫内膜癌肿瘤细胞的生长。
Objective：To explore the effect of mifepristone on apoptosis, cell cycle and subtypes of progesterone receptor in patients with endometrial carcinoma.Methods：60 samples were extracted before and after treatment of mifepristone, the rate of cell apoptosis and changes of cell cycle were detected by flow cytometry, the expression levels of PR-A mRNA and PR-B mRNA were detected by real-time quantitative PCR.Results：After treated with mifepristone, the apoptosis rate of cancer cells and ratio of G0/G1 increased, the ratio of S phase decreased, the expression of PR-B mRNA and the ratio of PR-B/PR-A decreased significantly.Conclusion：Mifepristone can down-regulate the expression of PR-B, block cancer cells at G1 phase, promote apoptosis and inhibit the growth of endometrial carcinoma cells.
出处
《中国妇幼保健》
CAS
北大核心
2010年第19期2640-2642,共3页
Maternal and Child Health Care of China
基金
广东省科技厅科研经费资助〔83098〕
关键词
米非司酮
子宫内膜癌
凋亡
细胞周期
孕激素受体
Mifepristone
Endometrial carcinoma
Apoptosis
Cell cycle
Progesterone receptor
