原发性肝细胞癌不同部位肿瘤浸润性淋巴细胞得率及成活率

The acquired number and activity of tumor infiltrating lymphocytes in different places in hepatocellular carcinoma tissue

目的探讨原发性肝细胞癌（ｐｒｉｍａｒｙｈｅｐａｔｏｃｅｌｕｌａｒｃａｒｃｉｎｏｍａ，ＰＨＣＣ）肿瘤组织不同部位肿瘤浸润性淋巴细胞（ｔｕｍｏｉｎｆｉｌｔｒａｔｉｎｇｌｙｍｐｈｏｃｙｔｅｓ，ＴＩＬ）的得率和成活率，以便制备ＴＩＬ用于过继免疫治疗时正确切取标本。方法本实验对１２例ＰＨＣＣ肿瘤组织不同部位ＴＩＬ的得率和成活率进行了比较。结果肿瘤周围组织和肿瘤边缘组织ＴＩＬ得率和成活率最高，肿瘤实质组织次之，肿瘤中心坏死组织最低，正常肝脏组织、腹水、胆管癌栓内几乎无ＴＩＬ。结论制备ＰＨＣＣ组织ＴＩＬ时，切取肿瘤周围组织和肿瘤边缘组织效果较好。

Objective To study the acquired number and activity of tumor infiltrating lymphocytes (TIL) from different places in primary hepatocellular carcinoma (PHCC) tissue. Methods The TILs acquired number and activity derived from different places of PHCC tissue in 12 cases were compared. Results The highest TILs acquired number and activity were gained from the superficial and peripheral tumor tissue; but the acquired number and activity were much lower in parenchyma tissue and center tissue of the tumor. There were almost no TILs in normal hepatic tissue, ascites and carcinomatous embolism of bile duct. Conclusions This study suggests that it be better to cut peripheral and superficial tumor tissue for gainings of TILs.