有氧运动对酒精性脂肪肝大鼠模型的影响

Effects of aerobic exercise on the rat model with alcoholic fatty liver

目的观察中等强度有氧运动对大鼠酒精性脂肪性肝病模型各种指标变化的影响,探讨运动对酒精性脂肪性肝病的作用。方法 4周龄健康雄性SD大鼠60只,分成空白对照组、模型对照组与造模运动组3组,每组20只。空白对照组:注射等量0.9%氯化钠溶液,不进行跑台运动训练。模型对照组:给予乙醇+高脂饮食灌胃,放进静止的跑台不运动,0坡度,30min/d。造模运动组:给予乙醇+高脂饮食灌胃,进行跑台运动训练,跑速30m/min,15%坡度,30min/d。运动持续4周。主要观察指标检测各组肝脏组织中光镜观察组织学、丙二醛、超氧化物歧化酶含量的变化。结果模型对照组肝细胞变性程度显著重于空白对照组(P<0.05),造模运动组肝细胞变性程度轻于模型对照组(P<0.05),但仍然比空白对照组严重(P<0.05)。空白对照组丙二醛含量显著低于模型对照组和造模运动组(P<0.05);造模运动组低于模型对照组(P<0.05)。空白对照组超氧化物歧化酶活性显著高于模型对照组和造模运动组(P<0.05),造模运动组超氧化物歧化酶活性高于模型对照组(P<0.05)。结论有氧运动能够对酒精性脂肪肝的功能恢复产生促进作用,降低对肝脏的损伤,但无法逆转脂肪肝的形成。

Objective To observe the index changes of rat models with alcoholic fatty liver after the aerobic exercise, in order to explore the effect of aerobic exercise on alcoholic fatty liver disease. Methods A randomized control animal trial was completed in the experiment. A total of 60 healthy male SD rats （4-week age） were divided into three groups in the experiment, with 20 ones in each group. The rats in blank control group were injected with saline and the rats didn＇ t undergo running training. The rats in model control group were fed with ethanol and pingnid food by intragastric garage and were put on the static treadmill of 0 slope, with the frequency of 30min/d. The rats in the modeling exercise group were fed with ethanol and pingnid food by intragastric gavage, undergoing treadmill exercise at a speed of 30m/min for 4 weeks, with the slope of 15% and the frequency of 30min/d. The histological changes were observed with light microscope. The contents of superoxide dismutases （SOD） and malonaldehydes （ MDA ） in the rat lever tissues were detected. Results The degree of liver cell degeneration in model control group was significantly higher than that of blank control group （ P 〈 0.01 ）, the degeneration of liver cells in the modeling exercise group was obviously lighter than that in model control group （ P 〈 0.05 ）, but still much severer than that in blank control group （ P 〈 0.01 ）. The MDA content in blank control group was markedly lower than that either in model control group or in modeling exercise group （ P 〈 0.05 ） ; the MDA content in modeling exercise group was significantly lower than that in model control group （ P 〈 0.05 ）. Furthermore, the SOD activity in blank control group was obviously higher than that either in model control group or in modeling exercise group （ P 〈 0.01 ）. The SOD activity in modeling exercise group was significantly higher than that in model control group （ P 〈 0.01 ）. Conclusion The aerobic exercise is able to encourage the functional recovery of alcoholic fatty liver and to limit its harm to livers. But it can＇ t change the formation of the alcoholic fatty liver disease .