铬胁迫对拟穴青蟹体内磷酸酶活性的影响

The Effects of Chromium Stress on the Activities of ACP and AKP in Different Tissues and Organs of Mud Crab Scylla paramamosain

采用生态毒理学方法,研究了水体中Cr6+(0.5,1.0,2.0,4.0,8.0 mg/L)胁迫1,3,5,7,9 d后对拟穴青蟹鳃、肌肉和肝胰腺中磷酸酶活性的影响,以未添加Cr6+的自然海水组为对照组.结果表明,Cr6+胁迫1 d后拟穴青蟹鳃中酸性磷酸酶(ACP)活性显著升高(p<0.05),肝胰腺、肌肉中ACP活性升高不显著(p>0.05),Cr6+胁迫9 d后,2.0,4.0,8.0mg/L Cr6+浓度组拟穴青蟹肝胰腺中ACP活性显著降低(p<0.05).5 d后不同Cr6+浓度组拟穴青蟹鳃中碱性磷酸酶(AKP)活性一直显著升高(p<0.05),胁迫9 d后,0.5,1.0,2.0 mg/L Cr6+浓度组与对照组差异不显著(p>0.05),4.0,8.0 mg/L Cr6+浓度组鳃中AKP活性仍显著高于对照组(p<0.05).2.0,4.0,8.0 mg/L Cr6+浓度组肝胰腺、肌肉中AKP活性在Cr6+胁迫1 d后显著升高(p<0.05).Cr6+胁迫显著影响拟穴青蟹生理生化效应.

A eco-toxicological experiment was conducted to investigate the effects of different concentrations of water-borne Cr^6＋ （0.5,1.0,2.0,4.0,8.0 mg/L）stress and nature seawater（don＇t contain Cr^6＋ , as the control） on the activities of ACP and AKP in gill, hepatopancreas and muscle of mud crab Scylla paramamosain. The present results indicated that the ACP activity in gill of S. paramamosain was increased significantly after 1 d Cr^6＋ exposure （p〈（0.05）, wherease, the ACP activity in hepatopancreas and muscle did not show significant changes （p〉0.05）. The ACP activity in hepatopancreas was significantly decreased （p〈0.05） after 9 d Cr^6＋ exposure at the concentrations of 2.0,4.0 and 8.0 rag/L, respectively. The AKP activity in gill was significantly increased （p〈0. 05） after 5 d Cr^6＋ exposure,and it did not show significant changes （p〉0.05） after 9 d Cr^6＋ exposure at the concentrations of 5,1.0 and 2.0 mg/L, respectively. On the other hand, the AKP activity in hepatopanereas and muscle was significantly increased （p〈0. 05） after 1 d Cr^6＋ exposure at the concentrations of 2.0,4.0 and 8.0 mg/L, respectively. Thus, chromium stress can significantly affect the physiological-biochemical parameter of Scylla paramamosian.