中草药提取物复配化妆品乳液延缓皮肤衰老实验研究

Anti-aging Effects of Skin Care Emulsion Mixed with Chinese Herbal Extracts

目的研究当归等5味中草药提取物复配护肤品乳液的抗皮肤衰老作用。方法将60只12个月龄雌性昆明种小鼠随机分成3组(n=20),自然衰老正常对照组(N组)、低剂量中草药提取物组(L组)、高剂量中草药提取物组(H组);另将20只3个月龄小鼠组成年青对照组(Y组)。L组用4%中草药提取物复配的化妆品乳液外涂,H组用16%中草药提取物复配的化妆品乳液外涂,N组和Y组用不含中草药提取物成分的化妆品基质乳液外涂。连续涂抹60 d,取小鼠背部涂抹部位皮肤,测定皮肤组织匀浆中的羟脯氨酸(HYP)、超氧化物歧化酶(SOD)及过氧化脂质(LPO)代谢产物丙二醛(MDA)、脂褐质(LF)的含量。结果 L组SOD活力较Y组明显下降,而MDA、LF的含量明显增加。L组及H组小鼠皮肤匀浆中的HYP含量分别达到(30.85±3.71)和(35.46±3.54)mg.g-1,SOD含量分别达到(17.92±0.58)和(20.38±0.71)U.mL-1,MDA含量达到为(6.92±1.38)和(5.96±1.25)mmo.lg-1,LF含量达到(6.13±1.73)和(4.89±1.15)μg.g-1;与对照组比较,均差异有显著性(P<0.05)。结论当归等5味中药提取物复配护肤品乳液具有较显著的抗皮肤衰老作用。

Objective To study the anti-aging effects of skin care emulsion mixed with five kinds of Chinese herbal extracts（CHE）. Methods Sixty female mice of 12 months were randomly divided into 3 groups（n=20）： normal aging control group（N）,low dose（L） and high dose（H） of CHE groups.Twenty other female mice of 3 months were used as young control groups（Y group）.Skin care emulsion with 4% CHE was besmeared on the skin of mice in L group,that with 16% CHE was besmeared on H group,and cosmetic matrix without CHE was besmeared on N and Y groups for 60 d.The dorsal skin samples were collected to determine the contents of hydroxyproline（HYP）,superoxide dismutase（SOD）,malondialdehyde（MDA） and lipofuscin（LF）. Results The SOD activity of natural aging group（N group） decreased more significantly,and the contents of MDA and LF increased obviously than those in the Y group,which showed that the natural aging model was successfully set up.The content of HYP in L and H groups reached to（30.85±3.71） and（35.46±3.54） mg·g-1,SOD were（17.92±0.58） and（20.38±0.71） U·mL-1,MDA were（6.92±1.38） and（5.96±1.25） mmol·g-1,LF were（6.13±1.73） and（4.89±1.15） μg·g-1,respectively,which were significantly different from those of the control group（P0.05）. Conclusion The skin care emulsion with five CHE including angelicae sinensis has a noticeable anti-skin aging effect.