摘要
应用Plackett-Burman设计法对影响纤维素酶液体发酵的培养基组分进行筛选,以纤维素粉、豆饼粉、KH2PO4、(NH4)2SO4、尿素、MgSO4、CaCl2和吐温-80为相关因素分析。确定了影响纤维素酶活力的关键因素为纤维素粉、豆饼粉和(NH4)2SO4。采用响应面法对影响纤维素酶活力的关键因素最佳水平范围进一步研究,并通过二次方程回归求解确定纤维素粉、豆饼粉和(NH4)2SO4的浓度分别为18.18 g/L、10.58 g/L和2.03 g/L时,模型预测的纤维素酶FPA活力为2.23 IU/mL,实验验证结果为2.19 IU/mL,预测值与验证值吻合较好。
The Plackett-Burman design was used to evaluate the effects of eight variables including cellulose, soybean meal, KH2PO4, （NH4）2SO4, urea, MgSO4, CaCl2 and Tween-80 on liquid fermentation of cellulase. By analyzing the statistical regression, cellulose, soybean meal and （NH4） 2SO4 were found to be the most important factors.Subsequently, response surface methodology was employed to determine the maximum ceUulase activity at optimum concentration of key factors. The maximum cellulase activity was correlated to the three parameters that could be represented by second-order polynomial models. The optimum values of the three parameters were determined as 18.18 g/L cellulose,10.58 g/L soybeanmeal and 2.03 g/L （NH4）2SO4. The activity of FPA was 2.23 IU/mL and the actual experimental result was 2.19 IU/mL. The predictive value was in good agreement with the actual value.
出处
《酿酒科技》
2010年第7期42-45,共4页
Liquor-Making Science & Technology
