人重组白细胞介素-6对大鼠海马神经元Bcl-2表达的影响

Effects of RhIL-6 on Bcl-2 Expression in Rat Hippocampal Neurons

观察重组人白细胞介素－6（rhIL－6）对体外培养大鼠海马神经元Bcl－2表达的影响。方法取培养3、7、14、21和28天（d）的两组（对照组和rhIL－6组）培养神经元，分别观察其生长发育和神经元活存数，并用抗Bcl－2抗血清进行免疫组化染色，观察Bcl-2免疫反应（Bcl-2－IR）阳性和阴性神经元数目，计算Bcl-2－IR阳性神经元所占百分率，并在图像分析仪上对Bcl-2－IR神经元作平均光密度的色谱分析。结果培养3、7、14、21和28d时，rhIL－6组神经元活存数、Bcl－2－IR阳性神经元数和Bcl-2-IR阳性神经元的平均光密度均明显高于对照组。结论rhIL－6能增强生长发育过程中海马神经元Bcl-2的表达，减少神经元的退化死亡，表明rhIL-6对体外培养的海马神经元具有神经营养作用。

To study the effects ofrecombinant human interleukin－6（RhIL－6） on Bcl－2 expression in cultured hippocampal neurons． Metthods The hippocampal neurons were im－munocytochem stained using the antiserum of Bcl－2 in 3、7、14、21 and 28 day old cell． The number and the mean optical density of Bcl－2－immunoreactive（Bcl－2－IR） neurons were observed． Results The number and the mean optical density of Bcl－2－IR neurons were significantly increased with rhIL－6 in cultured hippocampal neurons． Conclusion RhIL－6 can increased Bcl－2 expression and decreased death of cultured hippocampal neurons in vitro． It suggested that rhIL－6 mayact as a neurotrophic factor in the growth and development of hippocampal neurons．