几种环境因子对大弹涂鱼精子活力的影响

Effects of Environmental Factors on Vitality of Sperm in Boleophthalmus pectinirostris

通过测定精子的激活率、运动时间及寿命研究了盐度、pH、离子及葡萄糖等因子对大弹涂鱼精子活力的影响.结果表明：大弹涂鱼精子在盐度10－25范围内,精子激活率≥（81.67±5.69）%,其中盐度为20时精子活力最好,精子激活率、运动时间和寿命分别达（94.33±4.04）%、（87.67±8.02）min及（115.33±9.24）min;在pH6.0-9.0范围内精子激活率≥（80.33±4.51）%,运动时间≥（63±4）min,寿命≥（80±4.36）min,其中pH为8时,精子活力最好.NaCl浓度在150mmol·L^-1、KCl及MgCl2浓度在300mmol·L^-1、CaCl2及NaH2PO4浓度在100mmol·L^-1及葡萄糖浓度在400mmol·L^-1时,精子激活率较高.

The effects of pH value,salinity,ions and glucose solutions on the sperm vitality of Boleophthalmus pectinirostris are investigated through determination of activation ratio,moving duration and life-span of sperm. The results indicate that the activation ratio of sperm exceeds（81.67±7.21）%in the solutions with salinity ranging from 10 to 25.The highest activation ratio,the longest moving duration and life-span of sperm are found to be（94.33±4.04）%,（87.67±8.02）min and（115.33±9.24）min,respectively,in the case that the sperm is activated in the suitable solutions with salinity 20.Also,in the solutions of pH value 6.0-9.0,the activation ratio, the longest moving duration and life-span of sperm are all greater than（80.33±4.51）%,（63±4）min and（80±4.36） min respectively.The vitality reaches its maximum in the solutions of pH 8.The sperm has a greater activation ratio in the solutions with ions concentrations of NaCl,KCl,MgCl2,CaCl2,NaH2PO being 150,300,300,100, 100 mmol·L^- 1,respectively.