摘要
通过测定精子的激活率、运动时间及寿命研究了盐度、pH、离子及葡萄糖等因子对大弹涂鱼精子活力的影响.结果表明:大弹涂鱼精子在盐度10-25范围内,精子激活率≥(81.67±5.69)%,其中盐度为20时精子活力最好,精子激活率、运动时间和寿命分别达(94.33±4.04)%、(87.67±8.02)min及(115.33±9.24)min;在pH6.0-9.0范围内精子激活率≥(80.33±4.51)%,运动时间≥(63±4)min,寿命≥(80±4.36)min,其中pH为8时,精子活力最好.NaCl浓度在150mmol·L^-1、KCl及MgCl2浓度在300mmol·L^-1、CaCl2及NaH2PO4浓度在100mmol·L^-1及葡萄糖浓度在400mmol·L^-1时,精子激活率较高.
The effects of pH value,salinity,ions and glucose solutions on the sperm vitality of Boleophthalmus pectinirostris are investigated through determination of activation ratio,moving duration and life-span of sperm. The results indicate that the activation ratio of sperm exceeds(81.67±7.21)%in the solutions with salinity ranging from 10 to 25.The highest activation ratio,the longest moving duration and life-span of sperm are found to be(94.33±4.04)%,(87.67±8.02)min and(115.33±9.24)min,respectively,in the case that the sperm is activated in the suitable solutions with salinity 20.Also,in the solutions of pH value 6.0-9.0,the activation ratio, the longest moving duration and life-span of sperm are all greater than(80.33±4.51)%,(63±4)min and(80±4.36) min respectively.The vitality reaches its maximum in the solutions of pH 8.The sperm has a greater activation ratio in the solutions with ions concentrations of NaCl,KCl,MgCl2,CaCl2,NaH2PO being 150,300,300,100, 100 mmol·L^- 1,respectively.
出处
《宁波大学学报(理工版)》
CAS
2010年第3期11-15,共5页
Journal of Ningbo University:Natural Science and Engineering Edition
基金
宁波市科技计划项目(2006C100044
2007A31004)
浙江省科技计划项目(2005C32020)
关键词
大弹涂鱼
精子
活力
环境因子
Boleophthalmus pectinirostris
sperm
vitality
environmental factors