P21-ras、P53、Ki-67蛋白的表达与星形细胞瘤分化及预后的相关性研究

The correlation between expression of P21-ras P53 protoin Ki-67 and cell differentiation and prognosis in astrocytic neoplasms.

目的分析Ｐ２１－ｒａｓ、Ｐ５３、Ｋｉ－６７在星形细胞肿瘤中的表达，与肿瘤增生活性、分化和预后的关系。方法应用免疫组化ＬＳＡＢ法对６６例星形细胞瘤Ｐ２１－ｒａｓ、Ｐ５３、Ｋｉ－６７表达进行检测，对其中４７例有随访资料的肿瘤患者存活因素进行分析。结果Ｐ５３、Ｋｉ－６７的表达与肿瘤分级密切相关（Ｐ＜０．０１）。Ｐ５３、Ｋｉ－６７在肿瘤中的表达呈正相关（Ｐ＜０．０５）。Ｐ５３表达阳性，和Ｋｉ－６７表达指数高的肿瘤预后较差。Ｐ２１－ｒａｓ表达与肿瘤分化及预后未见显著差异。（Ｐ＞０．０５）。结论星形细胞瘤也存在Ｐ２１－ｒａｓ基因异常，Ｐ５３、Ｋｉ－６７能客观的反映肿瘤的增殖速度，分化和恶性程度，可以作为判断肿瘤预后的有用指标。

Objectives To study the correlation between expression of P21-rasP53 protein, Ki-67 and proliferating activity, cell differentiation and prognosis in astrocytic neoplasms. Methods Expression of P21-rasP53 protein. and Ki-67 in 66 cases of primary astrocytic neoplasms of the brain were evaluated with immunohistoc hemical LSAB method. Among 47 cases of tumor with followingup data, the expressions of P21-rasP53 protein and Ki-67 and surviving factors were synthetically analysed. Results The expression of P53 protein, and Ki-67 were closely correlated with the differentiation grade of neoplasms (F=12.83 P<0.01,F=34.74 P<0.01). The positive expression of P53 protein and higher indices of Ki-67 in neoplasms showed bad prognosis. The expression of p53 and Ki-67 showed positive correlative (r=0.37 P<0.05). The expression of P21-ras protein in tumor was no correlated with the differentiation grade of neoplasms and prognosis. Conclusions The results suggest that the expression of p53 and ki-67 were closely associated with prognosis of the neoplasms and they could objectively reflect to tumor proliferating, defferentiation and the degree of malignancy. There are H-ras mutant gene in the astrocytic neoplasms.