大鼠脊髓受压后血管通透性的变化和益气化瘀利水方药的作用

The Vascular Permeability Changes and the Effect of Recipe for Replenishing Qi, Removing Blood Stasis and Inducing Diureses

目的：观察血管通透性在脊髓受压后的改变和益气化瘀利水方药的药理机制。方法：通过注射１０６ Ｗ ａｌｋｅｒ ２５６ 瘤细胞液到大鼠 Ｔ１３ 椎体前，建立脊髓硬膜外压迫实验模型，通过伊文思蓝法定量测定脊髓血管通透性。结果：脊髓受压后血管通透性明显升高（ Ｐ＜ ０．０１），消炎痛和益气化瘀利水方药能明显抑制血管通透性，但消炎痛的作用强于益气化瘀利水方药（ Ｐ＜ ０．０１），颈痛灵对血管通透性则无影响（ Ｐ＞ ０．０５）。结论：脊髓受压后的水肿为血管源性水肿，益气化瘀利水方药通过减轻水肿，来延缓脊髓继发性损害的产生。

Objective:To clarify whether the change of vascular permeability in the spinal cord compressed segments and the effect of the recipe for replenishing Qi,removing blood stasis and inducing diureses on vascular permeability.Methods:The cord compression was induced im Wistar rats by percutaneous inoculation of 10 6 cells of walker 256 carcinoma cell suspension anterior to the T 13 vertebral body.Vascular permeability of the spinal cord was quantitated by measuring tissue staining with Evans blue dye.Results:In saline-treated compressed rats a signififcant increase in vascular permeability was present in the compressed segments (P<0.01).Treatment with either Indomethacin or the recipe for replenishing Qi,removing blood stasis and inducing diureses significantly reduced elevated vascular permeability when compared to saline-treated compressed rats (P<0.01),but the recipe for replenishing Qi,removing blood stasis and inducing diureses was significantly less potent than Indomethacin in reducing the increased vascular permeability.Jing Tong Ling failed to reduce the increased vascular permeability of the compressed segments (P>0.05).Conclusion:The spinal cord compressed causes vasogenic edema in the compressed segments.The recipe for replenishing Qi,removing blood stasis and inducing dirueses is effective in reducing spinal cord edema and protract the course to secondary damage of the cord.