慢性染铅对大鼠海马区神经细胞Ca^(2+)浓度及Ca^(2+)-ATP酶活性的影响

The effect of chronic lead exposure on hippocampal intraneuronal [Ca 2+ ] and activity of Ca 2+ ATPase in rats

目的：观察慢性染铅对大鼠海马区神经细胞Ｃａ２＋浓度及Ｃａ２＋－ＡＴＰ酶活性的影响。方法：用０．１５％醋酸铅饲养大鼠建立慢性染铅动物模型，参照Ｄｉｌｄｙ法和徐友涵法测定海马神经细胞Ｃａ２＋浓度及Ｃａ２＋－ＡＴＰ酶活性。结果发现：细胞内Ｃａ２＋浓度，染铅组（２０３．８３±３０．５０）ｎｍｏｌ／Ｌ，对照组（９７．６２±１９．８３）ｎｍｏｌ／Ｌ，ｔ＝８．３１Ｐ＜０．００５；Ｃａ２＋－ＡＴＰ酶活性，染铅组（３２６．４２±４０．０６）ｎｍｏｌ（Ｐｉ）·ｍｇ－１·ｍｉｎ－１，对照组（２５３．０７±２５．４０）ｎｍｏｌ（Ｐｉ）·ｍｇ－１·ｍｉｎ－１，ｔ＝３．５４，Ｐ＜０．０１。结论：慢性染铅可使大鼠海马区神经细胞内Ｃａ２＋浓度升高。

The objective of this experiment is to study the effect of lead on intracellular[Ca 2+ ] and the relationship between Ca 2+ level and Ca 2+ ATPase.Method:Chronic lead treated animal model is acquired by feeding the rats with 0 15% Pb(Ac) 2 in drinking water for 3 months.Intracellular[Ca 2+ ] and activity of Ca 2+ ATPase are determined with dildy and a respectively.Result:1.Intracellular[Ca 2+ ]lead treated group:203 83±30 50nmol/L control group:97 62±19 83nmol/L( P <0 005); 2.Ca 2+ ATPase activity:lead treated group:326 42±40 06nmol pi mg -1 min -1 ,control group:253 07±25 40nmol pi ng -1 min -1 ( P <0 01).Conclusion chronic leadexposure can significantly increase intracellular Ca 2+ level and activity of Ca 2+ ATPase.in hippocampal neurons. \ \