丹蒌片对高脂血症及血管内皮损伤大鼠的保护作用

Protection of Danlou Tablets on Hyperlipidemia and Vascular Endothelial Injury in Rats

目的研究丹蒌片对高血脂症大鼠血脂水平及高脂血症、血管内皮损伤大鼠血管内皮功能相关指标的影响。方法采用高脂饲料喂养所致大鼠高脂血症模型,测定血脂水平相关的指标血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、计算HDL/LDL比值,并测定与血管内皮损伤的相关指标内皮素(ET)、细胞间黏附分子-1(ICAM-1)、一氧化氮(NO)、降钙素基因相关肽(CGRP)、血栓素B2(TXB2)、6-酮-前列腺素F1α(6-keto-PGF1α)。采用机械性(导管牵拉)所致大鼠动脉内皮损伤模型,测定大鼠血清中一氧化氮合酶(NOS)、内皮素(ET)和血管紧张素Ⅱ(AngⅡ)的含量。结果丹蒌片能降低高脂饲料喂养所致高脂血症大鼠的血脂水平,能有效地改善血管内皮细胞功能相关指标;具有明显改善机械性损伤所致动脉内皮损伤大鼠血管内皮细胞功能相关指标的作用。结论丹蒌片能降低高脂血症大鼠血脂水平及改善动脉内皮损伤大鼠血管内皮功能。

Objective To study the impacts of danlou tablets on blood lipid level of the rats with hyperlipidemia and the relevant indexes of vascular endothelial function in the rats with hyperlipidemia and vascular endothelial injury.Methods The high-fat forage was used to induce hyperlipidemia models in rats.The indexes of blood lipid level（TC,TG,HDL-C,LDL-C,HDL/LDL）and the indexes of vascular endothelial injury（ET,ICAM-1,NO,CGRP,TXB2 and 6-keto-PGF1α）were determined in serum.The mechanical（catheter traction）method was adopted to establish arterial endothelial injury models in rats.The contents of NOS,ET and Ang-II were determined in serum.Results Danlou tablets decreased blood lipid of rats with hyperlipidemia induced by high-fat forage and improved the relevant indexes of vascular endothelial function effectively.The tablets improved apparently the relevant indexes of vascular endothelial function in the rats with arterial endothelial injury induced by mechanical injury.Conclusion Danlou tablets can reduce blood lipid level of the rats with hyperlipidemia and improve vascular endothelial function of the rats with arterial endothelial injury.