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人工杂交法获得拟南芥酯酶基因位点AT1G54790/AT2G429双突变体及其PCR鉴定与表型分析 被引量：1

The Acquisition of AT1G54790/AT2G42990 Double-Mutant Line and Characterization of the Mutant Phenotype
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摘要 以拟南芥T-DNA插入纯合突变体AT1G54790和AT2G42990为亲本,采用人工杂交技术与PCR鉴定相结合的方法获得了AT1G54790/AT2G42990纯合双突变体,并对表型进行了初步分析。 In this paper,the author crossed T-DNA inserted homozygous mutants AT1G54790 with AT2G42990 to produce AT1G54790/AT2G42990 double-mutant and used PCR technique to identify homogeneous double-mutant.In addition,the phenotype of homozygous double-mutant were analyzed.
作者 吴晓梅 陈明训
机构地区 丽水学院生物系 浙江大学作物科学研究所 浙江大学农业与生物技术学院
出处 《湖北农业科学》 北大核心 2010年第6期1285-1288,共4页 Hubei Agricultural Sciences
基金 国家自然科学基金项目(2006CB101602)
关键词 拟南芥 纯合双突变体 人工杂交 PCR 表型 Arabidopsis thaliana double-mutant cross PCR phenotype
分类号 Q753 [生物学—分子生物学]
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参考文献9

二级参考文献21

  • 1杨双,阮燕晔,樊金娟,张立军.一种简易的拟南芥幼苗微量DNA提取方法[J].沈阳农业大学学报,2005,36(1):99-100. 被引量：21
  • 2李敏,杨双,阮燕晔,樊金娟,张立军.拟南芥T-DNA插入突变体ATSUC3的PCR鉴定[J].植物生理学通讯,2006,42(1):91-94. 被引量：28
  • 3Barker L,Kühn C,Weises A,Schulz A,Gebhardt C,Hirner B,Hellmann H,Schulze W,Ward JM,Frommer WB (2000).SUT2,a putative sucrose sensor in sieve elements.Plant Cell,12:1153～1164.
  • 4Bouche N,Bouchez D (2001).Arabidopsis gene knockout:phenotypes wanted.Curr Opin Biotechnol,4:111～117.
  • 5Dreyer I,Horeau C,Lemaillet G,Zimmermann S,Bush DR,Rodriguez-Navarro A,Schachtman DP,Spalding EP,Sentenac H,Gaber RF (1999).Identification and characterization of plant transporters using heterogonous expression systems.J Exp Bot,50:1073～1087.
  • 6Meyer S,Melzer M,Truernit E,Hümmer C,Besenbeck R,Stadler R,Sauer N (2000).AtSUC3,a gene encoding a new Arabidopsis sucrose transporter,is expressed in cells adjacent to the vascular tissue and in a carpel cell layer.Plant J,24 (6):869～882.
  • 7Meyer S,Lauterbach C,Niedermeier M,Barth I,Sjolund RD,Sauer N (2004).Wounding enhanced expression of AtSUC3,a sucrose transporter from Arabidopsis sieve elements and sink tissues.Plant Physiol,134:684～693.
  • 8Parinov S,Sundaresan V (2000).Functional genomics in Arabidopsis:large-scale insertional mutagenesis complements the genome sequencing project.Curr Opin Biotechnol,11:157～161.
  • 9Schulze WX,Reinders A,Ward J,Lalonde S,Frommer WB (2003).Interactions between co-expressed Arabidopsis sucrose transporters in the split-ubiquitin system.BMC Biochem,4:3～12.
  • 10汤章城.现代植物生理学实验指南[M].北京：科学出版社,1999.12.

共引文献51

同被引文献10

  • 1田路明,黄丛林,张秀海,张潞生,吴忠义.逆境相关植物锌指蛋白的研究进展[J].生物技术通报,2005,21(6):12-16. 被引量：31
  • 2李敏,杨双,阮燕晔,樊金娟,张立军.拟南芥T-DNA插入突变体ATSUC3的PCR鉴定[J].植物生理学通讯,2006,42(1):91-94. 被引量：28
  • 3吴晓丹,张立军,白雪梅,李敏,阮燕晔.杂交方法获得的拟南芥蔗糖转运蛋白基因SUC3/SUC5双突变体及其PCR鉴定[J].植物生理学通讯,2006,42(6):1073-1076. 被引量：6
  • 4Takatsuji H. Zinc-ringer transcription tactors in plants[ J ]. Cellu- lar and Molecular Life Sciences, 1998,54(6) :582-596.
  • 5Murata T, Hikita K, Kaneda N. Transcriptional activation function of zinc finger protein TIS11 and its negative regulation by phorbol ester[ J]. Biochemical and Biophysical Research Communica- tions,2000,274(2) :526-532.
  • 6Doyle J J, Doyle J L. A rapid DNA isolation procedure for small quantities of fresh leaf tissueE J]. Phytochemical Bulletin, 1987, 19(1) :11-15.
  • 7Livak K J, $chmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-~acr Method[ J]. Methods ,2001,25 (4) :402-408.
  • 8Scong E S, Wang M H. A novel CaAbsil gone induced by early- abiotic stresses in pepper[ J]. Biochemistry and Molecular Biology Reports,2008,41 ( 1 ) :86-91.
  • 9Liu Qinglin, Xu Kedong, Ma Nan, et al. Isolation and functional characterization of DgZFP: a gene encoding a Cys2/His2-type zinc finger protein in chrysanthemum[ J]. Molecular Biology Re- ports,2010,37 (2) :1137-1142.
  • 10Sakamoto H, Maruyama K, Sakuma Y, et al. Arabidopsis Cys2/ His2-type zinc-finger proteins function as transcription repressors under drought, cold, and high-salinity stress conditions I J I- Plant Physiology,2004,136( 1 ) :2734-2746.

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湖北农业科学
2010年 第6期

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