uPA、uPAR在上皮性卵巢癌的表达及与MMPs相关性研究

Expression of uPA and uPAR in epithelial ovarian cancer and relationship between uPA/uPAR and MMPs

目的:探讨尿激酶型纤溶酶原激活物(uPA)及其受体(uPAR)在上皮性卵巢癌组织中的表达意义,研究uPA、uPAR与MMPs在肿瘤侵袭、转移中的相互关系。方法:采用免疫组化S-P法检测100例上皮性卵巢癌原发灶、30例相应的转移灶和20例正常卵巢组织中uPA、uPAR的表达;采用免疫荧光双染法检测uPA、uPAR与MMP-2、MMP-9在上皮性卵巢癌组织中的联合表达。结果:①uPA、uPAR在大多数上皮性卵巢癌原发灶及转移灶组织中均高表达,而在正常卵巢组织中不表达。②uPA和uPAR的高表达与肿瘤分化程度、临床分期、腹水及肿瘤复发显著相关(P<0.01),而与组织学类型及术后残留肿瘤无关(P>0.05)。③uPA、uPAR与MMPs在上皮性卵巢癌原发灶及转移灶组织中均联合表达。结论:uPA和uPAR在上皮性卵巢癌的发展和转移过程中起重要作用;uPA、uPAR与MMPs的相互作用可以促进肿瘤的生长与侵袭;uPA、uPAR可成为晚期、复发卵巢癌治疗的靶向目标。

Objective： To investigate the expression of uPA and uPAR in epithelial ovarian cancer （EOC） and the relationship between uPA/uPAR and MMPs. Methods： Expression of uPA and uPAR was detected using immunohistoehemical staining （SP） in EOC patients（ n = 100）, and matched metastatec lesions （ n = 30） and normal ovaries tissues （ n = 20） from untreated patients. Co- immunolabelling of uPA/uPAR and MMP -2, MMP -9 in EOC tissues was detected using immunofluorescence staining. Results ： （1） High expression of uPA and uPAR were observed in primary tumors and metastatic lesions in EOC,but not in normal ovarian tissues. （2） Over expression of uPA/uPAR in EOC was correlated significantly with progression parameters including tumor grade, relapse and ascites （P 〈 0. 01 ） , not correlated with histological type or residual tumor following surgery （P 〉0.05）. （3） Co - immunolabeling of uPA/uPAR and MMP was found in EOC specimens, but not in normal ovarian tissues. Conclusion： The results indicated that uPA and uPAR play an key role in EOC progression and metastasis. The interactions of uPA/uPAR and MMPs may promote EOC growth and invasion, which suggested that uPA/uPAR as potential therapeutic targets for treating late - stage recurrent metastatic EOC.