原蛋白转化酶在转化生长因子β_1抑制HBV复制效应中的作用

Effect of proprotein convertases on transforming growth factor β_1-induced HBV replication inhibition

目的:探讨原蛋白转化酶(PCs)在转化生长因子β1(TGFβ1)抑制HBV复制效应中的作用。方法:常规培养的HepG2.2.15细胞加2μg/L或5μg/L重组TGFβ1或/和20μmol/L PC抑制剂作用18h,收集细胞提取细胞总RNA和HBV衣壳DNA,采用荧光定量PCR技术检测PC mRNA和HBV DNA含量。结果:HepG2.2.15细胞的7种PCs均有不同程度的表达。加用重组TGFβ1后除PC5/6下调外,其余PCs均显著上调。尽管PC1/3和PC2上调最显著,但结合基础表达水平的分析显示furin和PACE4在TGFβ1处理前后均为优势表达PCs。在HepG2.2.15细胞上,进一步使用PC抑制剂可消除TGFβ1抑制HBV复制的效应。结论:上调PCs mRNA表达是TGFβ1抑制HBV复制的重要中间环节。此结果对进一步探讨TGFβ1有效干预HBV复制具有积极意义。

AIM ： To study the effect of proprotein eonvertases （PCs） on the transforming growth factor （TGF） β1 -induced inhibition of HBV replication. METHODS： HepG2. 2. 15 cells cultured regularly were exposed to recombinant TGFβ1 at concentration of 2 μg/L or 5 μg/L and/or PC inhibitor at concentration of 20 μmol/L for 18 h. The total RNA and HBV core particle DNA were extracted from these cells, and PC mRNA and core - associated HBV DNA were detected by real - time PCR technique. RESULTS ： The mRNA expression levels of 7 PCs in HepG2. 2. 15 cells were observed with various degrees. Recombinant TGFβ1 significantly up - regulated the mRNA expression of all PCs except for the down - regulation of PC5/6, though PC1/3 and PC2 were up - regulated most obviously. Furin and PACE4 were the predominant PCs before and after TGFβ1 exposure when the basic mRNA expression was taken into account. Further study showed that TGFβ1 - induced the inhibition of HBV replication was abrogated by PC inhibitor in HepG2. 2. 15 cells. CONCLUSION： TGFβ1 - induced the inhibition of HBV replication is mediated by the up - regulation of PCs, which might be of many implications in efficient interferences of TGFβ1 on HBV replication.