拟南芥血红蛋白1(AtGLB1)超量表达载体和RNAi表达载体的构建及转化

Construction and Transformation of Over-Expression Vector and RNAi Vector of AtGLB1

为研究拟南芥血红蛋白1基因(AtGLB1)的功能,将AtGLB1基因构建入超表达载体pMD和RNAi载体pZYI中,并采用花序浸染法将重组质粒转化拟南芥Columbia得到了转基因植株。对超表达株系和RNAi株系的纯合体进行Northern分析,结果表明:超量表达的拟南芥株系中AtGLB1的表达水平比野生型高很多,而表达被抑制的RNAi株系中几乎检测不到AtGLB1基因的表达。这些AtGLB1表达水平不同的拟南芥株系的获得可为该基因功能的后续研究以及在农业生产中的应用奠定一定基础。

The over-expression and RNAi constructs of AtGLB1 were constructed and transformed into Arabidopsis plants with floral dipping method.Northern blot analysis of AtGLB1 expression in homozygous T3 lines of transgenic Arabidopsis showed that AtGLB1 mRNA were not identified in lines expressing the AtGLB1 RNAi construct,while lines over-expressing AtGLB1 showed a substantially high level of AtGLB1 mRNA compared with the wild-type.The study laid foundation for further researches on the function and application of AtGLB1 gene in agriculture.