鲍曼不动杆菌斑点叉尾鮰株的分离与鉴定

Isolation and Identification of Acinetobacter Baumannii from the Diseased Channel Catfish

从湖北省宜昌市清江水库网箱养殖发病的斑点叉尾鮰肝脏分离到1株细菌CH016,该菌可在淡水琼脂平板上生长,单菌落圆形白色,表面圆滑,边缘整齐。经腹腔注射回归感染试验,复制出与自然发病相同的病症,且从人工感染病鱼体内再次分离到与自然发病相同的细菌,表明该菌对斑点叉尾鮰具有致病性。经生理生化鉴定、16S rRNA基因序列及其系统发育分析,结果均显示该菌为鲍曼不动杆菌。

Whereas it is well documented that Acinetobacter baumannii is associated with human clinical infections,there are few studies to date that reports it as a pathogen for aquatic animal.In the present study,one bacterial strain was isolated from diseased channel catfish（Ictalurus punctatus） from Qingjiang reservoir,Yichang,Hubei Province,China and named as CH016,which could be cultivated on freshwater agar and formed the round,white,smooth and margin-trim clone.Inoculating the strains to channel catfish for lethality indicated the germ had pathogenicity and the same bacterium could be isolated from these animals.The biochemical tests,the sequence analysis results of 16S rDNA sequence and phylogenetic analysis demonstrated that the microorganism isolated from channel catfish was A.baumannii.